Malaysian Journal of Analytical Sciences Vol 26 No 1 (2022): 96 - 108

 

 

 

 

ELICITATION OF INDUCED POLYKETIDE COMPOUNDS FROM A CO-CULTURE BETWEEN Streptomyces sp. STRAIN SUK10 AND Fusarium sp. AND THEIR ANTIBACTERIAL ACTIVITIES

 

(Elisitasi Sebatian Poliketida Teraruh daripada Satu Kultur-bersama di antara Streptomyces sp. Strain SUK10 dan Fusarium sp. dan Aktiviti Antibakteria)

 

Muhammad Asyraf Zawawi¹, Nurul Izzati Rosdi¹, Noor Wini Mazlan^1,2*, Mariam Taib¹, Kamariah Bakar², Noraziah Mohamad Zin³, Siti Nordahliawate M. Sidique⁴, Saif Aldeen Mohammad Fayiz Jaber⁵, RuAngelie Edrada-Ebel⁵

 

¹Faculty of Science and Marine Environment

²Institute of Marine Biotechnology

Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

³Center for Diagnostic, Therapeutic and Investigative Studies, Faculty of Health Sciences,

Universiti Kebangsaan Malaysia, Jalan Raja Muda Abd Aziz, 50300 Kuala Lumpur, Malaysia

⁴Faculty of Fisheries and Food Science,

Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

⁵Strathclyde Institute of Pharmacy and Biomedical Sciences,

University of Strathclyde, The John Arbuthnott Building, 161 Cathedral Street, Glasgow G4 0RE, Scotland

 

*Corresponding author:  noorwini@umt.edu.my

 

 

Received:  3 November 2021; Accepted: 30 December 2021; Published:  25 February 2022

 

 

Abstract

Endophytes including bacteria and fungi produce an array of biologically active secondary metabolites. Different approaches have been applied in order to increase the probability production of new metabolites including mimicking the environment, media and the microbes. However, the use of single culture usually re-produces known compounds with known bioactivities. Therefore, co-culturing between Streptomyces sp. strain SUK10 and Fusarium sp. in the same media at different growth stages leads to direct interaction which may trigger the expression of "silent" biosynthetic pathway to produce novel secondary metabolites. In this study, we elicited the production of the unknown secondary metabolites from co-culture extracts by using high resolution liquid chromatography-mass spectrometry, while data was processed by utilizing the quantitative expression analysis software MZmine 2.40.1 and SIMCA P+ 15.0 coupled with macro analysis and supported with DNP database for dereplication studies. The results showed that only the extract from co-culture of F7S15 showed enhances antibacterial activity on the Gram-positive bacteria with minimum inhibition concentration (MIC) values of 5 mg/mL and 10 mg/mL against Micrococcus sp. and Staphylococcus aureus, respectively, compared with their independent and other co-culture extracts were non-active. However, all extracts were non-active on the Gram-negative bacteria. Our preliminary results showed that the potential of co-culture method leading the production of novel metabolites which could be explored for future antibacterial agents.

 

Keywords:  co-culture, metabolomics, liquid chromatography-mass spectrometry, multivariate analysis, dereplication

 

Abstrak

Endofit termasuk bakteria dan fungus menghasilkan satu tatasusunan metabolit sekunder yang mempunyai keaktifan biologi. Pelbagai pendekatan telah digunakan bagi tujuan meningkatkan kebarangkalian penghasilan metabolit baru termasuk mengajuk persekitaran, media dan mikrob. Walaubagaimanapun, penggunaan kultur tunggal selalumya menghasilkan semula sebatian dengan bioaktiviti yang telah diketahui. Oleh itu, satu pengkulturan bersama di antara Streptomyces sp. strain SUK10 dan Fusarium sp. di dalam media yang sama di peringkat pertumbuhan yang berbeza, memacu kepada interaksi yang mungkin pencetus kepada ungkapan laluan biosintetik senyap untuk menghasilkan metabolit sekunder baru. Dalam kajian ini, kami telah memperolehi penghasilan metabolit sekunder yang belum diketahui daripada ekstrak kultur bersama dengan menggunakan kromatografi cecair-spektrometri jisim resolusi tinggi, sementara itu data telah dianalisis menggunakan perisian analisis ungkapan kuantitatif MZmine 2.40.1 dan SIMCA P+ 15.0 beserta analisis makro dan disokong oleh pengkalan data DNP bagi kajian dereplikasi. Keputusan telah menunjukkan bahawa hanya ekstrak daripada kultur bersama F7S15 telah menunjukkan peningkatan aktiviti antibakteria ke atas bakteria Gram-positif dengan nilai minimum kepekatan perencatan (MIC) 5 mg/mL dan 10 mg/mL melawan Micrococcus sp. dan Staphylococcus aureus, masing-masing, berbanding ekstrak kutur bebas dan kultur bersama yang lain adalah tidak aktif. Walaubagaimanapun, semua ekstrak tidak aktif ke atas bakteria Gram-negatif. Keputusan awal kajian kami telah menunjukkan potensi kaedah kultur bersama memacu penghasilan metabolit baru yang boleh diterokai bagi agen antibakteria masa hadapan.

 

Kata kunci:  kultur bersama, metabolomik, kromatografi cecair-spektrometri jisim, analisis multivariat, dereplikasi

 

 

Graphical Abstract

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