Malaysian Journal of Analytical Sciences Vol 24 No 6 (2020): 893 - 905

 

 

 

 

DEVELOPMENT OF DISPERSIVE MICRO-SOLID PHASE EXTRACTION FOR THE ANALYSIS OF OFLOXACIN AND SPARFLOXACIN IN HUMAN PLASMA

 

(Pembangunan Pengekstrakan Fasa Pepejal-Mikro Disesarkan untuk Analisis Oflosaksin dan Sparflosaksin dalam Plasma Manusia)

 

Sohaib Jumaah Owaid¹, Noorfatimah Yahaya², Nurul Yani Rahim¹, Rania Adam Edrees Mohammad¹, Maizatul Najwa Jajuli¹, Mazidatulakmam Miskam¹*

 

¹School of Chemical Sciences,
Universiti Sains Malaysia, 11900 Minden, Pulau Pinang, Malaysia

² Integrative Medicine Cluster, Advanced Medical and Dental Institute (AMDI),

Universiti Sains Malaysia, 13200 Bertam, Pulau Pinang, Malaysia

 

*Corresponding author:  mazidatul@usm.my

 

 

Received: 13 August 2020; Accepted: 18 October 2020; Published:  10 December 2020

 

 

Abstract

Dispersive micro-solid phase extraction (D-μ-SPE) using C₁₈ adsorbent combined with HPLV-UV was developed for the determination of ofloxacin and sparfloxacin in human plasma. Seven D‐μ‐SPE parameters namely type and amount of adsorbent mass, sample volume, pH of sample solution, extraction time, desorption solvent and volume were optimized. Under optimum conditions, calibration curves showed good linearity in the range of 0.5–1000 μg L^-1 with acceptable limit of detection (LOD) of 0.73 and 1.81 μg L^-1 and limit of quantitation (LOQ) of 2.44 and 6.03 μg L^-1 for ofloxacin and sparfloxacin, respectively. The D‐μ‐SPE also demonstrated acceptable precision at the concentration of 500 dan 1000 μg L^-1 of ofloxacin and sparfloxacin, respectively in human plasma with RSD value of ≤12.5%. A good relative recoveries was obtained between 90.1-109.5%. The developed D-μ-SPE method has proven to be a fast and simple approach which only requires low amount of extraction solvent for drug analysis.

 

Keywords: dispersive micro-solid phase extraction, high performance liquid chromatography, ofloxacin, sparfloxacin, human plasma 

 

Abstrak

Pengekstrakkan fasa pepejal mikro disesarkan (D-µ-SPE) menggunakan penjerap C₁₈ yang digabungkan dengan HPLC-UV telah dibangunkan untuk penentuan oflosaksin dan sparflosaksin dalam plasma manusia. Beberapa parameter D‐μ‐SPE seperti jenis dan jumlah jisim penjerap, isipadu sampel, pH larutan sampel, masa pengekstrakan, pelarut penyahjerap dan masa penyahjerap telah dioptimumkan. Dalam keadaan yang optimum, lengkung penentu ukuran menunjukkan lineariti yang baik dalam julat 0.5-1000 μg L^-1 dengan had pengesanan (LOD) 0.73 dan 1.81 μg L^-1 dan had pengukuran (LOQ) 2.44 dan 6.03 μg L^-1 yang memuaskan masing-masing bagi ofloksaksin dan sparflosaksin. Kaedah yang dicadangkan juga menunjukkan ketepatan pada kepekatan 500 dan 1000 μg L^-1 yang baik bagi oflosaksin dan sparflosaksin dari plasma manusia dengan RSD ≤12.5% ​​dan pemulihan relatif yang baik dalam julat 90.1-109.5%. Kaedah D‐μ‐SPE terbukti sebagai kaedah yang cepat danmudah untuk analisis ubat-ubatan kerana ia hanya memerlukan pelarut organik dalam jumlah yang kecil sewaktu analisis dijalankan.

 

Kata kunci: pengekstrakkan fasa pepejal mikro disesarkan, kromatografi cecair berprestasi tinggi, oflosaksin, sparflosaksin, plasma manusia

 

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