Malaysian Journal of Analytical Sciences Vol 24 No 6 (2020): 970 - 977

 

 

 

 

PHYTOCHEMICAL ANALYSIS AND ISOLATION OF SECONDARY METABOLITES FROM Persea declinata

 

(Analisis Fitokimia dan Pengasingan Metabolit Sekunder Daripada Persea declinata)

 

Sani Aliyu^1,2, Norazah Basar¹*, Yakubu Rufai^1,3_, Shamsul Khamis⁴

 

¹ Department of Chemistry, Faculty of Science,

Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia

² Department of Chemistry,

Kaduna State University, P.M.B 2339, Tafawa Balewa Way, Kaduna, Nigeria

³Department of Chemistry,

Federal College of Education Okene, P.M.B 1062 Kogi State, Nigeria

⁴School of Environment and Natural Resources Sciences, Faculty of Science and Technology,

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

 

*Corresponding author:  norazahb@utm.my

 

 

Received: 18 July 2019; Accepted: 20 July 2020; Published: 10 December 2020

 

 

Abstract

Phytochemical analysis of Persea declinata leaves and stembark extracts exhibited the presence of cardiac glycosides, coumarins, reducing sugars, steroids, flavonoids, tannins, and phenolic compounds. Sesamin (1), g-sitosterol (2), and palmitic acid (3) were isolated for the first time from ethyl acetate extract of the stembark of P. declinata by chromatographic methods. Their structures were elucidated based on spectroscopic analysis such as infrared (IR), gas chromatography-mass spectroscopy (GC-MS), nuclear magnetic resonance (NMR), and also involved comparison with data from literatures.

 

Keywords:  Persea declinata, phytochemicals, sesamin, gamma sitosterol, palmitic acid

 

Abstrak

Analisis fitokimia ke atas daun dan kulit batang Persea declinata menunjukkan kehadiran sebatian glikosida kardiak, kumarin, gula penurun, steroid, flavonoid, tanin dan sebatian fenolik. Sesamin (1), g-sitosterol (2), dan asid palmitik (3) telah diasingkan buat pertama kali daripada ekstrak etil asetat kulit batang P. declinata menggunakan kaedah kromatografi. Struktur sebatian telah dicirikan berdasarkan analisis spektroskopi seperti inframerah (IR), kromatografi gas-spektroskopi jisim (GC-MS), resonans magnet nuklear (NMR) dan juga melibatkan perbandingan data dengan kajian lepas.

 

Kata kunci:  Persea declinata, fitokimia, sesamin, gamma sitosterol, asid palmitik

 

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