Malaysian
Journal of Analytical Sciences Vol 24 No 5
(2020): 800 - 809
EFFECT OF TIME AND TEMPERATURE ON REDUCED
GRAPHENE OXIDE (rGO) LAYER STABILITY AND CYCLIC VOLTAMMETRIC BEHAVIOUR OF
MODIFIED SCREEN-PRINTED CARBON ELECTRODE (mSPCE) FOR BIOSENSING PURPOSES
(Kesan Masa dan Suhu Pengeraman terhadap Kestabilan
Lapisan rGO dan Perlakuan Voltametri Berkitar Elektrod Karbon Tercetak
Terubahsuai (mSPCE) bagi Tujuan Penderiaan Bio)
Ahmad Farid Mohd Azmi1*,
Vayithiswary Kannan2,3, Nur Sabila Yasin2, Jahwarhar
Izuan Abdul Rashid1, Ainsah Omar4, Emee Marina Salleh2
1Centre
for Defence Foundation Studies
2Centre for Research Management and Innovation
3Faculty of Defence Science and Technology
4Faculty of Medicine and Defence Health
Universiti Pertahanan Nasional Malaysia, Sungai Besi Camp, 57000 Kuala Lumpur,
Malaysia.
*Corresponding
author: ahmad.farid@upnm.edu.my
Received: 13 November 2019;
Accepted: 3 September 2020; Published: 12 October 2020
Abstract
In recent times, electrochemical immunosensing
has emerged as a promising technique for a simple, portable, cost-effective,
and efficient detection of cortisol in biofluids. The 2D graphene nanosheet is
widely considered as a ‘wonder material’, attributing to its numerous
incredible qualities which are commonly used as nanomaterial in today’s
biosensing technology. In this study, the working condition of biosensor for
layer stability and cyclic voltammetric behaviour of modified screen-printed
carbon electrode (mSPCE) integrated with reduced graphene oxide (rGO) was
optimized. Two main parameters, i.e., incubation time and incubation
temperature were investigated to assess the stability of rGO layer on the
surface of modified electrode. After the modification of SPCE with rGO, the
anodic and cathodic peak currents were remarkably enhanced at peak potentials
0.04 V and 0.22 V for incubation time and 0 V and 0.23 V for incubation
temperature, respectively. The optimum condition of layer stability for the
developed rGO-mSPCE was achieved by incubating the rGO on the electrode surface
for 24 hours at 25 °C.
Keywords:
biosensor, graphene oxide, electrochemical,
screen printed carbon electrode, incubation
Abstrak
Sejak kebelakangan ini,
teknik pengesanan penderiaan imuno elektrokimia telah muncul sebagai
teknik yang berpotensi dalam mengesan kortisol secara ringkas, mudah alih dan
cekap. Lapisan nano grafin 2D adalah terkenal sebagai bahan yang menakjubkan
yang merujuk kepada kepelbagaian kualitinya yang luar biasa yang menjadikannya
sebagai nanomaterial yang kini selalu digunakan dalam teknologi penderiaan bio. Dalam kajian ini, kestabilan lapisan
grafin teroksida yang terturun (rGO) dan tindak balas voltametri berkitar di
atas permukaan elektrod karbon tercetak terubahsuai (mSPCE) telah dioptimumkan.
Dua parameter utama iaitu tempoh pengeraman masa dan tempoh pengeraman suhu
telah dikaji untuk menilai kestabilan lapisan rGO di atas permukaan elektrod
tersebut. Selepas pengubahsuaian SPCE dengan rGO, arus puncak katod dan anod
telah dipertingkatkan pada potensi puncak bernilai 0.04 V dan 0.22 V bagi
tempoh pengeraman masa serta pada potensi puncak 0 V dan 0.23 V bagi tempoh
pengeraman suhu. Keadaan optimum untuk kestabilan lapisan rGO-mSPCE yang telah
diubahsuai dicapai melalui pengeraman lapisan rGO pada permukaan elektrod
selama 24 jam pada suhu 25 °C.
Kata kunci: biosensor, grafin oxida,
elektrokimia, elektrod karbon tercetak terubahsuai, pengeraman
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