Malaysian Journal of Analytical Sciences Vol 24 No 3 (2020): 313 - 319

 

 

 

 

CYTOTOXIC ACTIVITY OF Erythrina fusca LOUR. LEAF, TWIG AND FLOWER EXTRACTS

 

(Aktiviti Sitotoksik Ekstrak Daun, Ranting, dan Bunga Erythrina fusca Lour.)

 

Adiez Sapura Azmi¹, Norina Abdul Rahim¹, Zuriati Zahari¹, Fatimah Salim^2,3*

 

¹Faculty of Applied Sciences,

Universiti Teknologi MARA, Shah Alam, 40450, Selangor, Malaysia
²Atta-ur-Rahman Institute for Natural Product Discovery (AuRIns),

Universiti Teknologi MARA Selangor Branch, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia.
³Centre of Foundation Studies,

Universiti Teknologi MARA Selangor Branch, Dengkil Campus, 43800 Dengkil, Selangor, Malaysia

 

*Corresponding author:  fatimah2940@uitm.edu.my

 

 

Received: 29 November 2019; Accepted: 2 April 2020; Published: 9 June 2020

 

 

Abstract

Erythrina fusca Lour. belongs to the legume family (Fabaceae) is a species of flowering tree, locally known as ‘chengkering’. The plant is traditionally used for reducing inflammation, relieving migraine, reducing skin irritation and for dressing fresh wounds. This work reports on cytotoxic activities of the methanolic leaf, twig and flower extracts of E. fusca Lour. against human liver cancer cell line (HepG2) and monkey kidney normal cell line (Vero). The extracts were found to be nontoxic on both cell lines with IC₅₀ values of more than 100 µg/mL and therapeutic index of less than 2. In order to obtain comparative information on the chemical composition and the complexity of the structure, the methanolic extracts were profiled on a reversed-phase ultrahigh-performance liquid chromatography (UHPLC).

 

Keywords:  Erythrina fusca Lour., cytotoxic activities, HepG2, vero, ultrahigh-performance liquid chromatography

 

Abstrak

Erythrina fusca Lour. berasal daripada famili legume (Fabaceae) merupakan spesies pokok berbunga dan nama tempatannya ialah chengkerng. Tumbuhan ini digunakan secara tradisional untuk mengurangkan keradangan, melegakan migrain, mengurangkan kegatalan kulit dan pembalut luka. Kajian ini melaporkan aktiviti sitotoksik ekstrak metanol daun, ranting dan bunga E. fusca Lour. terhadap sel tunggal kanser hati manusia (HepG2) dan sel tunggal buah pinggang monyet (Vero). Kesemua ekstrak didapati tidak toksik kepada kedua jenis sel tunggal yang diuji dengan nilai IC₅₀ lebih daripada 100 µg/mL dengan indeks teraputik kurang daripada 2. Bagi memperolehi data perbandingan mengenai komposisi kimia dan kerumitan dalam struktur mereka, kesemua ekstrak metanol ini telah diprofilkan dalam fasa terbalik kromatografi cecair berprestasi tinggi ultra (UHPLC).

 

Keywords:  Erythrina fusca Lour., aktiviti sitotoksik, HepG2, vero, kromatografi cecair berprestasi tinggi ultra

 

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