Malaysian Journal of Analytical Sciences Vol 24 No 1 (2020): 70 - 77

 

 

 

 

PHYTOCHEMICAL SCREENING AND FTIR SPECTROSCOPY ON CRUDE EXTRACT FROM Enhalus acoroides LEAVES

 

(Saringan Fitokimia dan Spektroskopi FTIR Ekstrak Mentah Daun Enhalus acoroides)

 

Made Pharmawati¹* and Luh Putu Wrasiati²

 

¹Biology Department, Faculty of Mathematics and Natural Sciences

²Department of Agro-Industrial Technology, Faculty of Agricultural Technology

Udayana University, Kampus Bukit Jimbaran, Badung, 80361, Bali, Indonesia

 

*Corresponding author:  made_pharmawati@unud.ac.id

 

 

Received: 25 November 2019; Accepted: 9 January 2020

 

 

Abstract

Seagrass provides key ecological services in marine ecosystems, such as stabilising sediment, providing oxygen and acting as a nursery ground for marine biota. Seagrass has also been reported to have antioxidant activity that is useful for humans. One seagrass species, Enhalus acoroides, is widely distributed in Indonesia. This study aims to screen the phytochemical compounds, determine the functional groups and evaluate the profile of pigments present in E. acoroides leaf extract, which were collected from Semawang Beach, Sanur, Bali, Indonesia. The leaf extract was prepared using chloroform: ethanol (9:1) and tested for the presence of saponin, phenols, tannins and flavonoids. The functional groups and pigment profile were determined via Fourier-transform infrared spectroscopy (FTIR) and thin-layer chromatography (TLC), respectively. The results showed that the E. acoroides leaf extract contained phenols, tannins and flavonoids. The major functional groups found in the leaf extract were hydroxyl groups, lipids, alkanes, secondary amines, fatty acids, benzenoid compounds and phenols. The FTIR analysis also identified the presence of chlorophyll and carotenoids in the extract, which was further supported by the TLC analysis. This research shows that E. acoroides is a potential source of antioxidants and provides an opportunity for the development of natural products from E. acoroides in drug discovery.

 

Keywords:  Enhalus acoroides, FTIR, chromatography, phytochemical compound, seagrass

 

Abstrak

Rumput laut memainkan peranan penting dalam ekosistem marin yang menstabilkan sedimen, membekalkan oksigen dan berfungsi sebagai halaman bagi biota marin. Rumput laut juga dilaporkan mengandungi aktiviti antioksidan yang bermanfaat bagi manusia. Spesis rumput laut, Enhalus acoroides di jumpai meluas di Indonesia. Kajian ini bertujuan menyaring sebatian fitokimia, penentuan kumpulan berfungsi dan menilai profil pigmen yang wujud di dalam ekstrak daun E. acoroides, yang diambil dari Pantai Semawang, Sanur, Bali, Indonesia. Ekstrak daun disediakan menggunakan klorofom: etanol (9:1) dan diuji untuk penentuan kehadiran saponin, fenol, tannin dan flavonoid. Kumpulan berfungsi dan profil pigmen telah ditentukan masing-masing melalui spektroskopi inframerah transformasi Fourier (FTIR) dan kromatografi lapisan nipis (TLC). Hasil kajian menunjukkan ekstrak daun E. acoroides mengandungi fenol, tannin dan flavonoids. Kumpulan berfungsi utama yang dijumpai dalam ekstrak daun adalah kumpulan hidroksil, lipid, alkana, amina sekunder, asid lemak, sebatian benzoid dan fenol. Analisis FTIR juga mengenalpasti kehadiran klorofil dan karotenoid di dalam ekstrak, yang mana ia juga disokong oleh hasil analisis TLC. This research shows that E. acoroides is a potential source of antioxidants and provides an opportunity for the development of natural products from E. acoroides in drug discovery. Hasil kajian mendapati E. acoroides berpotensi sebagai sumber antioksidan dan potensinya dalam pembangunan sumber semulajadi dalam penemuan ubat.

 

Kata kunci:  Enhalus acoroides, FTIR, kromatografi, sebatian fitokimia, rumput laut

 

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