Malaysian
Journal of Analytical Sciences Vol 24 No 1 (2020): 97 - 106
ELECTROCHEMICAL
DETERMINATION OF DOPAMINE AND URIC ACID IN
BLOOD SERUM USING ANIONIC SURFACTANTS AT
CARBON PASTE ELECTRODES
(Penentuan Dopamin dan Asid Urik dalam
Serum Darah Secara
Elektrokimia Menggunakan Surfaktan Anionik pada Perekat Elektrod Karbon)
Liridon Berisha1,
Egzontina Shabani1, Arsim Maloku1*, Granit Jashari2,
Tahir Arbneshi1
1Department of Chemistry, Faculty
of Mathematical and Natural Sciences,
University
of Prishtina, 10000 Prishtinë, Kosovo
2Department of Analytical Chemistry, Faculty
of Chemical Technology,
University
of Pardubice, Studentská 573, 532 10 Pardubice, Czech Republic
*Corresponding author: arsim.maloku@uni-pr.edu
Received: 20 October 2019;
Accepted: 9 December 2019
Abstract
A selective and
sensitive method was developed for the simultaneous electrochemical determination
of dopamine and uric acid by using a sodium dodecylbenzenesulfonate (SDBS) and
sodium dodecyl sulfate (SDS) as a surface modifier of carbon paste electrodes
(CPEs). At lower concentration of SDS and SDBS they form a negatively charged
monolayer on CPE surface because of surfactants’ hydrophobic chain interaction
with paraffin of CPE. Optimized concentration of surfactants was 2 mM for SDS
and 1 mM for SDBS in phosphate buffer solution (0.1 M, pH 7 and pH 6,
respectively). Compared with plain CPE, CPE modified with SDS (CPE-SDS) and CPE
modified with SDBS (CPE-SDBS) have shown the improved electrochemical response
of dopamine (DA) at 0.230 V and uric acid (UA) at 0.345 V due to electrostatic
interactions between positively charged analytes and surface negatively charged
of SDBS and SDS. Under optimal experimental conditions, the designed electrodes
exhibited a wide range of linear response to DA from 0.53 μM to 31.6
μM and UA from 5.95 μM to 118.97 μM. The detection limits for DA
and UA were found to be 0.26 and 1.10 µM with CPE-SDS, whilst 0.22 and 0.38 µM
with CPE-SDBS. The CPE-SDBS and CPE-SDS showed good reproducibility,
repeatability, stability and high selectivity for determination of DA and UA in
blood serum samples.
Keywords: dopamine, uric acid, carbon paste
electrode, sodium dodecyl sulfate, sodium dodecylbenzenesulfonate
Abstrak
Kaedah sensitif dan selektif telah dibangun untuk penentuan serentak
dopamin dan asid urik menggunakan sodium dodekilbenzenasolfonat (SDBS) dan
sodium dodekil sulfat (SDS) sebagai perekat elektrod karbon yang diubah
permukaan. Pada kepekatan rendah SDS dan
SDBS membentuk cas negatif lapisan mono pada permukaan CPE disebabkan interaksi
rantaian hidrofobik dengan paraffin CPE. Kepekatan optimum surfaktan ialah 2 mM
SDS dan 1 mM SDBS dalam larutan penimbal fosfat (masing-masing 0.1 M, pH 7
dan pH 6). Berbanding CPE kosong, CPE yang terubahsuai dengan SDS (CPE-SDS)
dan CPE terubahsuai dengan SDBS (CPE-SDBS) telah menunjukkan peningkatan
respons elektrokimia bagi dopamin (DA) pada 0.230 V dan asid urik (UA) pada
0.345 V disebabkan oleh interaksi elektrostatik di antara cas positif analit
dan cas negatif permukaan SDBS dan SDS. Pada keadaan optimum, elektrod yang
dibangun menghasilkan julat respons kelinearan yang besar terhadap DA dari 0.53
μM to 31.6 μM hingga UA dari 5.95 μM hingga 118.97 μM. Had
pengesanan DA dan UA didapati pada 0.26 dan 1.10 µM bagi CPE-SDS, manakala 0.22
and 0.38 µM bagi CPE- SDBS. CPE-SDBS dan CPE-SDS telah menghasilkan
kebolehhasilan, kebolehulangan, kestabilan yang baik dan kepilihan yang tinggi
bagi penentuan DA dan UA di dalam sampel serum darah.
Kata
kunci:
dopamin,
asid urik, perekat elektrod karbon, sodium dodekil sulfat, sodium
dodekilbenzenasolfonat
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