Malaysian Journal of Analytical Sciences Vol 23 No 3 (2019): 516 - 523

DOI: 10.17576/mjas-2019-2303-16

 

 

 

Kinetic Degradation of Total Phenolic Content, DPPH RadicAl Scavenging and Xanthine Oxidase Inhibitory ActivitIES in Yanang (Tiliacora triandra) Leaf Extract during preparation process

 

(Degradasi Kinetik Kandungan Jumlah Fenolik, Aktiviti Pemerangkapan Radikal DPPH dan Xantin Oksidase dalam Ekstrak Daun Yanang (Tiliacora triandra) Semasa Proses Penyediaan)

 

Sunee Eadmusik¹*, Chanthima Phungamngoen¹, Natthaya Choosuk²

 

¹Department of Agro-industry Technology and Management, Faculty of Agro-industry

²Department of Innovation and Product Development Technology, Faculty of Agro-industry

King Mongkut’s University of Technology North Bangkok, 25230 Prachinburi, Thailand

 

*Corresponding author:  sunee.e@agro.kmutnb.ac.th

 

 

Received: 25 October 2017; Accepted: 22 January 2019

 

 

Abstract

Presently, canned bamboo shoots in Yanang (Tiliacora  triandra (Colebr.) Diels) leaf extract is produced in order to expand shelf-life and ease to prepare food. The objectives of this research were to investigate the effect of different thermal processing during Yanang leaf extract preparation on total phenolic content, DPPH radical scavenging and xanthine oxidase inhibitory activities and to determine kinetics of the extract’s quality alterations. The preparation included 1:12 (w/v) leaves to water ratio, 32,000 rpm blending speed and different heating temperature at 60, 70 or 80 ^oC for 15 minutes. Data were collected at 0, 1, 3, 6, 9, 12 and 15 minutes of heating time. The results showed that fresh Yanang leaf extract had total phenolic content of 680.16 ± 19.57 mg GAE/100 mg db. and it possessed DPPH radical scavenging and xanthine oxidase inhibitory activities at 163.53 ± 0.87 µg BHT/mL and 78.58 ± 0.17%, respectively. The results revealed that an increase in heating temperature and time decreased total phenolic content and the DPPH radical scavenging and xanthine oxidase inhibitory activities of Yanang leaf extract. During 15 minutes heating, the total phenolic content of Yanang leaf extract ranged from 526.33 ± 28.79 to 638.93 ± 29.69 mg GAE/100 mg db., DPPH radical scavenging activity ranged from 92.86 ± 1.66 to 136.41 ± 5.70 µg BHT/mL and xanthine oxidase inhibitory activity ranged from 72.64 ± 0.34 to 76.65 ± 0.50%. The change of total phenolic content (R² = 0.809-0.959) followed zero-order kinetic model and that of DPPH radical scavenging activity (R²=0.783-0.920) while xanthine oxidase inhibitory activity (R² = 0.864-0.922) followed second-order kinetic model.

 

Keywords:  Yanang, Tiliacora triandra, total phenolic content, xanthine oxidase, kinetic model

 

Abstrak

Pada masa ini, pucuk buluh dalam ekstrak daun Yanang (Tiliacora triandra (Colebr.) Diels) yang ditinkan dihasilkan untuk meningkatkan hayat simpanan dan kemudahan untuk menyediakan makanan. Objektif penyelidikan ini adalah untuk mengkaji kesan pemprosesan terma yang berbeza semasa penyediaan ekstrak daun Yanang terhadap kandungan jumlah fenolik, aktiviti pemerangkapan radikal DPPH dan xantin oksidase dan untuk menentukan kinetik perubahan kualiti ekstrak. Penyediaan melibatkan nisbah daun kepada air, 1:12 (w/v) 32,000 rpm halaju adunan dan suhu pemanasan yang berbeza pada 60, 70 atau 80 ^oC selama 15 minit. Data dikumpulkan pada masa pemanasan 0, 1, 3, 6, 9, 12 dan 15 minit. Keputusan menunjukkan bahawa ekstrak daun Yanang segar mempunyai kandungan jumlah fenolik sebanyak 680.16 ± 19.57 mg GAE/100 mg berat kering dan aktiviti pemerangkapan radikal DPPH dan xantin oksidase ialah 163.53 ±  0.87 μg BHT/mL dan 78.58 ± 0.17%. Keputusan menunjukkan bahawa peningkatan suhu pemanasan dan masa menurunkan kandungan jumlah fenolik dan aktiviti ekstrak daun Yanang. Selepas 15 minit pemanasan, kandungan fenolik jumlah ekstrak daun Yanang ialah dari 526.33 ± 28.79 hingga 638.93 ± 29.69 mg GAE/100 mg berat kering, aktiviti perencatan radikal DPPH adalah daripada 92.86 ± 1.66 hingga 136.41 ± 5.70 μg BHT/mL dan aktiviti perencatan xantin oksidase dalam julat 72.64 ± 0.34 hingga 76.65 ± 0.50%. Perubahan kandungan fenolik jumlah (R² = 0.809-0.959) adalah mengikut model kinetik tertib sifar dan aktiviti pemerangkapan radikal DPPH (R² = 0.783-0.920) sementara aktiviti penghambatan xantin oksidase (R² = 0.864-0.922) adalah mengikut modeltertib kedua.

 

Kata kunci:  Yanang, Tiliacora triandra, kandungan jumlah fenolik, xantin oksidase, model kinetik

 

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