Malaysian Journal of Analytical Sciences Vol 23 No 3 (2019): 505 - 515

DOI: 10.17576/mjas-2019-2303-15

 

 

 

RAPID SPECTROPHOTOMETRIC METHOD FOR HISTAMINE DETERMINATION IN FISH USING ALIZARIN RED S AND METAL

 

(Kaedah Spektrofotometri Pantas untuk Penentuan Histamin dalam Ikan Menggunakan Alizarin Merah S dan Logam)

 

Miftakhul Jannatin¹, Ayu Nabila Izazi Latjuba¹, Sri Wahyuni¹, Ganden Supriyanto^1,2*, Wan Aini Wan Ibrahim³

 

¹Department of Chemistry, Faculty of Science and Technology

²Laboratory of Sensor and Biosensor, Institute of Tropical Disease

Universitas Airlangga, Jl. Mulyorejo Kampus C UNAIR Surabaya 60115, Indonesia

³Department of Chemistry, Faculty of Science,

Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia

 

*Corresponding author:  ganden-s@fst.unair.ac.id

 

 

Received: 25 October 2017; Accepted: 22 January 2019

 

 

Abstract

An analytical method for the determination of histamine using metals and alizarin red S (ARS) reagents by UV-Visible spectrophotometry was developed. Cu(II), Co(II) and ARS were used to form colored complex with histamine. The developed method was used to detect qualitatively and quantitatively the presence of histamine. Absorbance of Cu(II) and Co(II) complex were measured at a maximum wavelength of 505.5 and 567 nm, respectively. Optimization of analytical parameters such as concentration of metals, concentration of ARS, sample solution pH, and optimum time for complex formation were performed. Experimental results showed that the optimum concentration of Cu(II) was 50 ppm, 75 ppm ARS, sample solution pH 6, and 15 minutes optimum time, while the optimum concentration of Co(II) was 125 ppm, ARS was 75 and 50 ppm, sample solution pH 5 and 10 minutes optimum time. Method validation indicated that the coefficient of variation, limit of detection (LOD) (S/N =3), and limit of quantitation (LOQ = 10S/N) of Cu(II) were 0.65%, 8.94 ppm, and 29.82 ppm respectively with a sensitivity of 0.0054 ppm and linearity of 0.9959. Accuracy of histamine determination through Cu-ARS-histamine complex at a concentration of 50, 100, and 150 ppm were 105.7% (RSD = 0.777%, n = 3), 105.06% (RSD = 0.606%, n = 3), and 94.12% (RSD = 0.767%, n = 3), respectively. Meanwhile, the CV, LOD, and LOQ of Co(II) were 0.28%; 2.58 ppm; and 8.6 ppm respectively with a sensitivity of 0.0006 ppm and linearity of 0.9965. Accuracy of histamine determination through Co-ARS-histamine complex at a concentration of 50, 75, and 125 ppm were 98.33%, 95.83%, 93.88%, respectively. The proposed method was successfully applied to the determination of histamine in a fish sample with quantitative recovery; for Cu(II) complex (99.23%) and Co(II) complex (102.62%).

 

Keywords:  histamine, metals, Cu(II), Co(II), ARS, UV-visible spectrophotometry

 

Abstrak

Kaedah analisis untuk penentuan histamin menggunakan logam dan reagen alizarin merah S (ARS) telah dibangunkan menggunakan spektrofotometri ultralembayung-nampak. Cu(II), Co(II), dan ARS digunakan untuk membentuk kompleks berwarna dengan histamin. Kaedah yang telah dibangunkan digunakan untuk mengesan histamin secara kualitatif dan kuantitatif. Serapan kompleks Cu(II) dan Co(II) diukur pada panjang gelombang maksimum masing-masing 505.5 dan 567 nm. Pengoptimuman parameter analisis seperti kepekatan logam, kepekatan ARS, pH larutan sampel, dan masa optimum bagi pembentukan kompleks telah dilakukan. Keputusan eksperimen menunjukkan bahawa kepekatan optimum Cu(II) ialah 50 ppm, 75 ppm ARS, pH 6 larutan sampel, dan masa optimum 15 minit, manakala kepekatan optimum Co(II) ialah 125 ppm, ARS ialah 75 dan 50 ppm, pH larutan sampel 5 dan 10 minit masa optimum. Pengesahan kaedah menunjukkan bahawa pekali variasi (CV), had pengesanan (LOD) (S/N = 3) dan had kuantititatif (LOQ = 10 S/N) Cu(II) masing-masing adalah 0.65%, 8.94 ppm dan 29.82 ppm dengan sensitiviti 0.0054 ppm dan kelinearan 0.9959. Ketepatan penentuan histamin melalui kompleks Cu-ARS-histamin pada kepekatan 50, 100, dan 150 ppm ialah 105.7% (RSD = 0.777%, n = 3), 105.06% (RSD = 0.606%, n = 3), dan 94.12% (RSD = 0.767%, n = 3), sementara itu,CV, LOD, dan LOQ Co(II) masing-masing adalah 0.28%; 2.58 ppm dan 8.6 ppm dengan kepekaan 0.0006 ppm dan kelinearan 0.9965. Ketepatan penentuan histamin melalui kompleks Co-ARS-histamin pada kepekatan 50, 75, dan 125 ppm masing-masing adalah 98.33%, 95.83%, dan 93.88%. Kaedah yang dicadangkan berjaya digunakan untuk menentukan histamin dalam sampel ikan dengan pengembalian kuantitatif untuk kompleks Cu(II) (99.23%) dan kompleks Co(II) (102.62%).

 

Kata kunci:  histamin, logam, Cu(II), Co(II), alizarin merah S, spektrofotometri ultralembayung-nampak

 

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