Malaysian Journal of Analytical Sciences Vol 23 No 3 (2019): 376 - 383

Candida rugosa LIPASE IMMOBILIZED ON DIETHYLAMINOETHYL-CELLULOSE (DEAE) FOR ESTERIFICATION OF BUTYL OLEATE

(Lipase daripada Candida rugosa Tersekatgerak pada Dietilaminoetil-Selulosa (DEAE) untuk Penghasilan Ester Butil Oleat)

Mohd Basyaruddin Abdul Rahman^1,2*, Ruhil Naznin Azaman², Emmy Maryati Omar^1,2, Muhammad Alif Mohammad Latif^1,2, Emilia Abdulmalek^1,2

¹Integrated Chemical BioPhysics Research, Faculty of Science

²Department of Chemistry, Faculty of Science

Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

*Corresponding author: basya@upm.edu.my

Received: 19 August 2018; Accepted: 14 May 2019

Abstract

Lipase from Candida rugosa was immobilized onto diethylaminoethyl-cellulose (DEAE) through physical adsorption method with high percentage of protein absorption obtained at 84%. The lipase-DEAE was used to catalyze the enzymatic esterification of butyl oleate by reacting oleic acid and butanol in hexane. Butyl oleate, an alkylic ester of long-chain fatty acid is now in high demand to produce biodiesel. The effect of reaction temperature, thermostability of the immobilization lipase, stability in organic solvent, leaching with hexane and storage studies under various conditions of immobilized lipase were investigated. The optimum esterification was found to be up to 90% yield. Only a slight of lipase leached out after being washed by 20 mL of hexane. This showed that lipases were strongly attached to the DEAE support via physical adsorption method, and it could be used as industrial biocatalyst.

Keywords: immobilized enzyme, lipase, cellulose, esterification, biodiesel

Abstrak

Lipase dari Candida rugosa tersekatgerak pada dietilaminoetil-selulosa (DEAE) melalui kaedah penjerapan fizikal menunjukkan peratusan penyerapan protein tertinggi pada kadar 83.4%. Lipase-DEAE digunakan sebagai mangkin untuk sintesis berenzim butil oleat melalui tindak balas asid oleik dan butanol di dalam heksana. Butil oleat, satu ester alkilik dari asid lemak rantai panjang kini mempunyai permintaan tinggi untuk penghasilan biodiesel. Kesan suhu tindak balas, kestabilan terma lipase tersekatgerak, kestabilan pelarut organik, pelunturan dengan heksana dan kajian penyimpanan pada pelbagai kondisi lipase tersekatgerak telah diselidiki. Hasil tindak balas pada keadaan optima didapati menghampiri 90%. Hanya sedikit kuantiti lipase yang hilang selepas proses pelunturan setelah dicuci oleh pelarut heksana 20 mL. Ini menunjukkan bahawa lipase telah tersekatgerak dengan kuat pada bahan DEAE melalui kaedah penjerapan fizikal, dan ianya boleh digunakan sebagai biomangkin industri.

Kata kunci: enzim tersekatgerak, lipase, selulosa, esterifikasi, biodiesel

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