Malaysian Journal of Analytical Sciences Vol 23 No 2 (2019): 204 - 211

DOI: 10.17576/mjas-2019-2302-04

 

 

 

SOL-GEL/CHITOSAN HYBRID THIN FILM IMMOBILISED WITH CURCUMIN AS pH INDICATOR FOR pH SENSOR FABRICATION

 

(Filem Nipis Hibrid Sol-gel/Kitosan Terpegun dengan Kurkumin sebagai Penunjuk pH bagi Fabrikasi Sensor pH)

 

Rosmawani Mohammad^1,2* and Musa Ahmad^2,3

 

¹Faculty of Bioengineering and Technology,

Universiti Malaysia Kelantan, Jeli Campus, Locked Bag No. 100, 17600 Jeli, Kelantan, Malaysia

²School of Chemical Sciences and Food Technology, Faculty of Sciences and Technology,

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

³Industrial Chemical Technology Program, Faculty of Science and Technology,

Universiti Sains Islam Malaysia, Bandar Baru Nilai, 71800 Nilai, Negeri Sembilan, Malaysia

 

*Corresponding author:  rosmawani@umk.edu.my

 

 

Received: 10 October 2018; Accepted: 21 February 2019

 

 

Abstract

An optical pH sensor based on the immobilisation of curcumin in sol-gel/chitosan hybrid thin films was fabricated on a cellulose acetate sheet as solid support for preparing a thin film. The thin film was prepared using the dip coating method. This sensor gave a range of linear pH responses at pH 9-13 (R² = 0.9821). The relative standard deviation (RSD) values of repeatability of this sensor were 7.03% (pH 10) and 3.34% (pH 11), while the RSD values of reproducibility were 7.46% (pH 10) and 8.66% (pH 11). The photostability of immobilised curcumin gave an RSD value of 2.37% for a study period of 15 weeks. This sensor had a response time between 10-20 s, and no hysteresis effect was observed when the pH in a cycle of pH 2-12-2 was measured. This sensor was applied in the determination of pH in real samples, and the results were comparable to a conventional pH meter.

 

Keywords:  optical pH sensor, hybrid sol-gel/chitosan, curcumin, thin film

 

Abstrak

Sensor optik pH berdasarkan pemegunan kurkumin dalam filem nipis hibrid sol-gel / kitosan difabrikasi di atas kepingan selulosa asetat sebagai penyokong untuk penyediaan filem nipis. Filem nipis telah disediakan menggunakan kaedah penyalutan celup. Sensor ini memberikan julat rangsangan linear pH pada pH 9-13 (R² = 0.9821).  Nilai sisihan piawai relatif (RSD) bagi kebolehasilan sensor ini adalah  7.03% (pH 10) dan 3.34% (pH 11), manakala nilai RSD kebolehulangan ialah 7.46% (pH 10) dan 8.66% (pH 11). Kestabilanfoto reagen kurkumin terpegun memberikan nilai RSD 2.37% untuk tempoh kajian selama 15 minggu. Sensor ini mempunyai masa tindak balas antara 10-20 s dan tiada kesan  histerisis diperhatikan apabila  sensor  digunakan  untuk  mengukur pH dalam kitaran pH 2-12-2. Sensor ini digunakan untuk penentuan pH dalam sampel sebenar dan hasilnya dapat dibandingkan dengan meter pH konvensional.

 

Kata kunci:  sensor pH optik, sol-gel hibrid / kitosan, kurkumin, filem nipis

 

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