Malaysian Journal of Analytical Sciences Vol 22 No 2 (2018): 197 - 202

DOI: 10.17576/mjas-2018-2202-04

 

 

 

VALIDATION METHOD FOR EXTRACTION AND DETERMINATION OF MARBOFLOXACIN IN PLASMA AND EDIBLE CHICKEN TISSUES

 

 (Kaedah Validasi bagi Pengesktrakan dan Penentuan Marbofloxacin di dalam Plasma dan Tisu Ayam yang Boleh Di Makan)

 

Natalia Francisca Urzúa¹*, Guillermo Fermín Prieto¹, Carlos Fernando Lüders², Maria Paula Tonini¹,

Eduardo Jesús Picco³, Carlos Alberto Errecalde¹

 

¹ Department of Animal Clinic, Faculty of Agronomy and Veterinary Medicine,

University National of Rio Cuarto, Route 36 Km 601, Rio Cuarto, Argentina

² School of Veterinary Medicine,

Catholic University of Temuco, Casilla 15D, Temuco, Chile

³ Chair of Pharmacology, Faculty of Veterinary Sciences,

University National of the Litoral, Kreder 2805, Esperanza, Argentina

 

^*Corresponding author:  nurzuapizarro@ayv.unrc.edu.ar

 

 

Received: 16 December 2017; Accepted: 16 February 2018

 

 

Abstract

Marbofloxacin (MBX) is an antimicrobial for exclusive veterinary use, relatively new and of which there are few analytical methods for the extraction, identification and quantification in plasma and edible tissues (muscle, liver, kidney, lung, skin) in broiler chickens. The objective of developing a simple, sensitive and efficient extraction and detection method through high-performance liquid chromatography (HPLC) with fluorescence detection. For the preparation of the samples, known concentrations of MBX, enrofloxacin (ENX) as internal standard, methanol, water and perchloric acid, were added to plasma or tissues. Chromatographic conditions were: fluorescence detector at λex 295 and λem 490 nm, mobile phase composed of deionized water, acetonitrile and triethylamine adjusted to pH 3.0. For all matrices, the method was linear (r²= 0.99) for MAR concentrations (0.039 to 2.5 μg/ml or gr), with an average retention time for MBX in the different matrices of 3.97 ± 0.144 minutes, and 4.99 ± 0.24 minutes for the internal standard (enrofloxacin). Detection limit in all matrices was less than 0.006 μg/ml, while recoverability percentage was 89 ± 4.65% and variation coefficients in intraday-interday assay ≤ 1.4%, determining the accuracy of the study. The analytical method applied is efficient, sensitive and reliable for future residue determinations or pharmacokinetic studies of marbofloxacin in plasma, muscle, liver, kidney, skin and lung in broiler chickens.

 

Keywords:  marbofloxacin, high-performance liquid chromatography, chicken

 

Abstrak

Marbofloxacin (MBX) ialah antimikrob yang digunakan secara eksklusif bagi veterinar, secara relatifnya adalah baru dan hanya beberapa kaedah analisis bagi pengekstrakan, pengenalpastian dan kuantifikasi di dalam plasma dan tisu yang boleh di makan (otot, hati, buah pinggang, paru-paru dan kulit) di dalam ayam daging telah di perkenal. Objektif kajian adalah pembangunan kaedah pengesanan yang mudah, sensitif dan berkesan menggunakan kromatografi cecair berprestasi tinggi (HPLC) dengan pengesan pendaflour. Bagi penyediaan sampel, kepekatan MBX, enrofloxacin (ENX) sebagai piawai dalam, metanol, air dan asid perklorik ditambah ke dalam tisu plasma. Tetapan kromatografi adalah: pengesan pendaflour pada λex 295 dan λem 490 nm, fasa bergerak terdiri daripada air ternyahion, asetonitril, dan trietilamina yang diselaraskan pada pH 3.0. Bagi semua matriks sampel, kaedah adalah bersifat linear (r²= 0.99) bagi kepekatan MBX (0.039 hingga 2.5 μg/ml atau μg/g), dengan purata masa tahanan di dalam matriks berbeza ialah 3.97 ± 0.144 minit, dan 4.99 ± 0.24 minit bagi piawai (enrofloxacin). Had pengesan di dalam semua matriks adalah lebih rendah dari nilai 0.006 μg/ml, manakala peratus perolehan semula ialah 89 ± 4.65% dan pekali variasi bagi ujian intra-hari dan inter-hari ≤ 1.4%, menjelaskan kejituan kaedah. Kaedah analisis yang digunapakai adalah efisien, sensitif, dan dipercayai bagi tujuan penentuan sisa atau kajian farmakokinetik marbofloxacin di dalam plasma, otot, hati, buah pinggang, kulit dan paru-paru ayam daging.

 

Kata kunci:  marbofloxacin, kromatografi cecair berprestasi tinggi, ayam

 

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