Malaysian Journal of Analytical Sciences Vol 26 No 2 (2022): 215 - 228

 

 

 

 

PREPARATION AND ADSORPTION STUDIES OF MOLECULARLY IMPRINTED POLYMER FOR SELECTIVE RECOGNITION OF TRYPTOPHAN

 

(Penyediaan dan Kajian Penjerapan Polimer Molekul Tercetak untuk Pengecaman Selektif Triptofan)

 

Nur Habibah Safiyah Jusoh¹, Faizatul Shimal Mehamod²*, Noor Fadilah Yusof³, Abd Mutalib Md Jani⁴, Faiz Bukhari Mohd Suah⁵, Marinah Mohd Ariffin¹, Nur Asyiqin Zulkefli¹

 

¹Faculty of Science and Marine Environment

²Advanced Nano Materials (ANoMA) Research Group, Faculty of Science and Marine Environment

Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

³School of Chemical and Energy Engineering, Faculty of Engineering,

Universiti Teknologi Malaysia, 81310 Skudai, Johor Bahru, Malaysia.

⁴Faculty of Applied Sciences,

Universiti Teknologi MARA, Perak Branch, Tapah Campus, 35400 Perak, Malaysia

⁵School of Chemical Sciences,

Universiti Sains Malaysia, 11800 Minden, Pulau Pinang, Malaysia

 

*Corresponding author:  fshimal@umt.edu.my

 

 

Received: 13 September 2021; Accepted:  18 December 2021; Published:  28 April 2022

 

 

Abstract

One of the effective technologies in molecular recognition is based on the molecular imprinting process. In this work, the polymers were prepared by bulk polymerization, using methacrylic acid and ethylene glycol dimethacrylate as the functional monomer and crosslinking agent, respectively. The polymers were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy and surface area and porosity analyses. Several parameters influencing the adsorption efficiency of Tryptophan (Tryp) such as adsorbent dosage, contact time, pH of sample solution as well as selectivity and reproducibility study, have been evaluated. The Tryptophan-imprinted polymer (Tryp-IP) showed significantly higher removal efficiency and selective binding capacity towards Tryp compared to non-imprinted polymer (NIP). The adsorption isotherm demonstrated that the Tryp-IP followed Langmuir isotherm model, indicating the Tryp-IP owning the homogenous surface type of adsorbent. In contrast, the NIP fit with the Redlich-Peterson model, indicating that mechanism adsorption is a mixed type. The kinetic study revealed that pseudo-second order was the appropriate kinetic model for Tryp-IP and the adsorption kinetic of NIP appeared to fit with pseudo-first order.

 

Keywords:  molecularly imprinted polymer, tryptophan, adsorption study

 

Abstrak

Salah satu teknologi yang efektif dalam pengecaman molekul adalah berdasarkan proses pencetakan molekul. Dalam kajian ini, polimer telah disediakan melalui pempolimeran pukal, masing-masing menggunakan asid metakrilik dan etilena glikol dimetakrilat sebagai monomer berfungsi dan agen tautsilang. Polimer dicirikan oleh spektroskopi inframerah transformasi Fourier, pengimbasan mikroskop elektron dan analisis luas permukaan dan keliangan. Beberapa parameter yang mempengaruhi kecekapan penjerapan Triptofan (Tryp) seperti dos penjerap, masa sentuhan, pH larutan sampel serta kajian selektiviti dan kebolehulangan telah dinilai. Polimer tercetak-Triptofan (Tryp-IP) menunjukkan kecekapan penyingkiran dan kapasiti pengikatan yang lebih tinggi terhadap Tryp berbanding polimer tidak dicetak (NIP). Isoterma penjerapan menunjukkan bahawa Tryp-IP mematuhi model isoterma Langmuir, ini menunjukkan Tryp-IP memiliki jenis penjerap permukaan homogen. Sebaliknya, NIP mematuhi model Redlich-Peterson, menunjukkan mekanisme penjerapan adalah jenis campuran. Kajian kinetik mendedahkan bahawa tertib pseudo-kedua adalah model kinetik yang sesuai untuk Tryp-IP dan kinetik penjerapan NIP kelihatan lebih sesuai dengan tertib pseudo-pertama.

 

Kata kunci:  polimer tercetak molekul, triptofan, kajian penjerapan

 

 

 

Graphical Abstract

 

 

 

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