Malaysian Journal of Analytical Sciences Vol 25 No 4 (2021): 661 - 668

 

 

 

 

CHEMICAL COMPOSITION OF KASTURI TOBACCO RESINOID DETERMINED BY GAS CHROMATOGRAPHY-MASS SPECTROMETRY

 

(Komposisi Sebatian Kimia Resinoid Tembakau Kasturi Menggunakan Kromatografi Gas-Spektrometri Jisim)

 

Larissa Tania^1,5, Ari Satia Nugraha^2,5, Tri Handoyo^3,5, Banun Kusumawardani^4,5*

 

¹Graduate School of Biotechnology, Postgraduate Program,

²Drug Utilisation and Discovery Research Group, Faculty of Pharmacy,

³Department of Agronomy, Faculty of Agriculture,

⁴Department of Biomedical Sciences, Faculty of Dentistry,

⁵Center for Development of Advance Science and Technology,

University of Jember, Jember 68121, East Java, Indonesia

 

*Corresponding author:  banun_k.fkg@unej.ac.id

 

 

Received: 19 July 2021; Accepted: 8 August 2021; Published:  29 August 2021

 

 

Abstract

In Indonesia, Kasturi tobacco is widely cultivated in Jember and Bondowoso Districts. This local tobacco planted in dry season and sundried processed which the leaves were used as the raw material of premium cigarettes. The leaves constitute of diverse molecules with various biological or pharmacological activities that have been widely used in medical treatments in form of natural based medicament. This study aimed to determine the chemical composition in the Kasturi tobacco leaves using gas chromatography-mass spectrometry (GC-MS). Kasturi tobacco leaves were air-dried prior extraction using stirred assisted maceration method for 24 hours in methanol. Less polar components was extracted using n-hexane and vacuum dried before fractionation under a silica column chromatography. Fractions were analyzed using gas chromatography-mass spectrometry resulted in detection of esters, hydrocarbons, alcohol, nitrogenous compounds and terpenoids including, two unique tobacco resinoids α-CBT (4,8,13-cyclotetradecatriene-1,3-diol) and the labdanoid (11E, 13Z)-labdadien-8-ol and (12Z)-abienol.

 

Keywords:  diterpenoid, fractionation, gas chromatography-mass spectrometry, Kasturi tobacco leaves, resinoid

 

Abstrak

Di Indonesia, tembakau Kasturi ditanam secara meluas di daerah Jember dan Bondowoso. Tembakau tempatan ini di tanam pada musim kering dan proses pengeringan di mana daun digunakan sebagai bahan mentah dalam pembuatan rokok premium. Bahagian daun yang kaya dengan aktiviti biologi dan farmakologi telah digunakan secara meluas bagi rawatan perubatan dalam bentuk ubatan semulajadi. Kajian ini bertujuan mengkaji komposisi kimia di dalam daun tembakau Kasturi menggunakan kromatografi gas-spektrometri jisim (GC-MS). Daun tembakau Kasturi terlebih dahulu dikeringkan sebelum pengekstrakan kaedah maserasi berbantukan pengacauan selama 24 jam di dalam larutan metanol. Sebatian tak berkutub diekstrak mengunakan n-heksana dan pengeringan vakum sebelum pemisahan dilakukan melalui kromatografi turus silika. Hasil analisis kromatografi gas-spektrometri jisim mengesan kehadiran ester, hidrokarbon, alkohol, sebatian nbernitrogen dan terpenoids termasuklah resinoids unik iaitu α-CBT (4,8,13-siklotetradekatrien-1,3-diol) and the labdanoid (11E, 13Z)-labdadien-8-ol and (12Z)-abienol.

 

Keywords:  diterpenoid, pemisahan, kromatografi gas-pektrometri jisim, daun tembakau Kasturi, resinoid

 

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