Malaysian Journal of Analytical Sciences Vol 25 No 1 (2021): 71 - 80

 

 

 

 

MEFENAMIC ACID DETERMINATION IN TABLET FORMULATIONS USING A SELECTIVE AND ACCURATE SPECTROPHOTOMETRIC METHOD BASED ON PRUSSIAN BLUE FORMATION

 

(Penentuan Asid Mefenamik dalam Formulasi Tablet Menggunakan Kaedah Spektrofotometri yang Selektif dan Tepat Berdasarkan Pembentukan Biru Prusian)

 

Hendri Wasito^1,2*, Desi Purnamasari², Muhamad Salman Fareza¹

 

¹Department of Pharmacy,

Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto 53123, Indonesia

²Analytical Chemistry for Pharmaceutical and Health Research Group, Department of Pharmacy, Faculty of Health Sciences,

Jenderal Soedirman University, Purwokerto 53123, Indonesia

 

^*Corresponding author:  hendri.wasito@unsoed.ac.id

 

 

Received: 10 November 2020; Accepted: 6 January 2021; Published:  20 February 2021

 

 

Abstract

Selective and accurate visible spectrophotometric methods based on Prussian blue formation were successfully developed for the quantitative analysis of mefenamic acid in a tablet. The proposed method showed that visible spectrophotometric measurement at 715 nm under the optimized condition of 15 mmol/L potassium ferricyanide and 2.5 mmol/L ferric chlorides in 0.5 mol/L acetic acids after 15 minutes incubation time. The analytical performance demonstrated good linearity with the squares of correlation coefficient (r²) more than 0.99 in the concentration ranges of 3.0-14.0 mg/L with a limit of detection and quantification (LOD and LOQ) were found to be 0.31 mg/L and 1.04 mg/L, respectively. The method also seemed to be selective that proven by small interference with various excipients added. The developed and validated method was suitable for routine analysis of mefenamic acid in real samples of the tablet.

 

Keywords:  quantification, mefenamic acid, spectrophotometry, validation

 

Abstrak

Kaedah spektrofotometri kelihatan selektif dan tepat berdasarkan pembentukan biru Prusian berjaya dibangunkan untuk analisis kuantitatif asid mefenamik dalam penyediaan tablet. Kaedah yang dicadangkan menunjukkan bahawa pengukuran optimum spektrofotometri yang dapat dilihat pada 715 nm di bawah keadaan 15 mmol/L kalium feriksianida dan 2.5 mmol/L ferik klorida dalam 0.5 mol/L asid asetik setelah 15 minit masa inkubasi. Prestasi analitikal menunjukkan kelinearan yang baik dengan pekali korelasi (r²) lebih besar daripada 0.99 dalam julat kepekatan 3.0-14.0 mg/L dengan had pengesanan dan kuantifikasi (LOD dan LOQ) masing-masing didapati 0.31 mg/L dan 1.04 mg/L. Kaedah ini juga nampaknya selektif yang terbukti dengan campur tangan kecil dengan pelbagai eksipien yang ditambahkan. Kaedah yang dibangunkan dan disahkan sesuai untuk analisis rutin asid mefenamik pada sampel sebenar tablet.

 

Kata kunci:  kuantifikasi, asid mefenamik, spektrofotometri, pengesahan

 

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