Malaysian Journal of Analytical Sciences Vol 24 No 3 (2020): 449 - 463

 

 

 

 

STANDARIZATION BASED ON CHEMICAL MARKERS OF Moringa oleifera HERBAL PRODUCTS USING BIOAUTOGRAPHY ASSAY, THIN LAYER CHROMATOGRAPHY AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-DIODE ARRAY DETECTOR

 

(Piawaian Berdasarkan Penanda Kimia bagi Produk Herba Moringa oleifera Menggunakan Ujian Bioautografi, Kromatografi Lapisan Nipis dan Kromatografi Cecair Berprestasi Tinggi-Pengesan Susunan Diod)

 

Erick Sierra-Campos¹*, Mónica A. Valdez-Solana¹, Claudia I. Avitia-Domínguez², Alfredo Téllez-Valencia², Jorge A. Meza-Velázquez¹, Miguel Aguilera-Ortiz¹, Adelma Escobar-Ramírez³

 

¹Facultad de Ciencias Químicas GP,

Universidad Juárez del Estado de Durango,  Av. Artículo 123 S/N Fracc. Filadelfia. Gómez Palacio, Durango. México

²Facultad de Medicina y Nutrición,

 Universidad Juárez del Estado de Durango, Av. Universidad y Fanny Anitúa S/N, 34000 Durango. México

³División Académica de Ciencias Básicas,

Universidad Juárez Autónoma de Tabasco,  Av. Universidad S/N, Zona de la Cultura, Col. Magisterial, Vhsa, Centro, Tabasco, México

 

*Corresponding author:  ericksier@ujed.mx

 

 

Received: 29 December 2019; Accepted: 25 April 2020; Published:  9 June 2020

 

 

Abstract

The present study investigated the variation of phytochemical composition, antioxidant activity and chemometric thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) fingerprinting profiles of five Moringa oleifera herbal products. TLC and HPLC along with the PCA and K-mean can adequately discriminate different herbal products. The content of total bioactive compounds of the samples were ranged from 591.5 to 966.1 mg/100 g dried weight. The total antioxidant activity of M. oleifera samples was in the following order: Mas lait > De Font Quer > Akuanandi > Infusionate > Terbal.  Wavelength at 352 nm, 75 components were detected in all herbal products; among these, 10 potential biomarkers with higher contents enabled differentiation between each sample and foreign organic matter. HPLC-DAD analysis of the extracts showed a decline in the quality of components in the five commercial products.  Thus, this study presents the TLC and HPLC profiles of the Mexican M. oleifera extracts that may serve as a reference for the proper identification, standardization and quality control of this plant. These methods can be used for routine quality control of herbal products and formulations containing this medicinal plant.

 

Keywords:  Moringa oleifera leaves, chemical markers

 

 

Abstrak

Kajian ini menyiasat variasi komposisi fitokimia, aktiviti antioksidan dan kemometrik kromatografi lapisan nipis (TLC) dan kromatografi cecair berprestasi tinggi (HPLC) bagi profil pencapjarian lima produk herba berasaskan Moringa oleifera. TLC dan HPLC bersama PCA dan K-min boleh membezalayan secara tepat bagi produk berbeza. Jumlah kandungan sebatian bioaktif bagi sampel berada di dalam julat 591.5 to 966.1 mg/100 g berat kering. Jumlah aktiviti antioksidan bagi sampel M. oleifera seperti tertib berikut: Mas lait > De Font Quer > Akuanandi > Infusionate > Terbal. Para panjang gelombang 352 nm, 75 komponen ditemui dalam semua produk herba; antaranya 10 potensi penanda bio dengan kadungannya yang tinggi membantu membezakan setiap sampel dengan jirim organik asing. Analisis HPLC-DAD terhadap ekstrak menunjukkan penurunan dalam kualiti komponen di dalam lima produk komersial. Kajian ini membentangkan profil TLC dan HPLC bagi ekstrak M. oleifera tulen dari Mexico yang boleh digunakan sebagai rujukan bagi tujuan pengenalpastian, paiwaian dan kawalan kualiti tumbuhan ini. Keadah ini boleh digunakan bagi rutin kawalan kualiti produk herba dan formulasi yang mengandungi tumbuhan perubatan ini.

 

Kata kunci:  daun Moringa oleifera, penanda kimia

 

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