Malaysian Journal of Analytical Sciences Vol 24 No 3 (2020): 436 - 448

 

 

 

 

GREEN SYNTHESIZED COPPER NANOPARTICLES FROM Blumea balsamifera LINN. LEAVES AND ITS BIOCIDAL ACTIVITIES AGAINST Bactrocera dorsalis (HENDEL)

 

(Sintesis Hijau Nanopartikel Kuprum dari Daun Blumea balsamifera Linn dan Aktiviti Biosida Melawan Bactrocera dorsalis (Hendel))

 

Danila S. Paragas ^1,2*, Kathlia D.C. Cruz¹, and Elaida R. Fiegalan³

 

¹School of Chemical, Biological and Materials Engineering and Sciences,

Mapua University, Intramuros, Manila, Philippines

²Department of Chemistry, College of Arts and Sciences,

Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines

³Department of Crop Protection, College of Agriculture,

Central Luzon State University, Science City of Muñoz, Nueva Ecija, Philippines

 

*Corresponding author:  danilaparagas1010@clsu.edu.ph

 

 

Received: 20 November 2019; Accepted: 16 April 2020; Published:  9 June 2020

 

 

Abstract

The biocidal components of sambong (Blumea balsamifera) leaves against oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), were extracted using four green solvents, namely rice wine, rice wash, apple cider vinegar, and distilled water employing hot infusion, maceration, fermentation, and Soxhlet methods. These extracts were used in the synthesis of copper nanoparticles (CuNPs). The suspensions of nanoparticles were centrifuged, and the aggregates were separated and dried. The dried aggregates and colloidal suspensions of copper nanoparticles were tested on male adult oriental fruit flies. Improvised olfactometer was used to determine the adulticidal activities of the nanoparticles. Both dried aggregates and colloidal suspensions had varying effects on the mortality rates of fruit flies, which ranged from 25-100% within 12 hours exposure. However, dried aggregates yielded higher mortality rates than those of the colloidal suspensions. The researchers concluded that sambong can be used in the preparation of nanoparticle-enhanced biocide either as suspension concentrate or dust or powder with suitable sorption material. Ultimately, sambong can be considered as an alternative in managing the oriental fruit fly.

 

Keywords:  biocide, Blumea balsamifera, nanoparticle, nanosuspension, oriental fruit fly

 

Abstrak

Komponen biosida bagi daun sambong (Blumea balsamifera) melawan lalat buat oriental, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), telah diekstrak menggunakan empat pelarut hijau iaitu wain beras, basuhan beras, cuka epal, dan air suling berdasarkan keadah infusi panas, maserasi, penapaian dan Soxhlet. Pengestrakan ini digunakan dalam sistesis nanopartikel kuprum (CuNPs). Nanopartikel terampai telah diempar, dan gumpalan telah diasing dan dikeringkan. Gumpalan kering dan ampaian koloid bagi nanopartikel kuprum telah diuji terhadap lalat buah oriental jantan dewasa. Olfactometer telah digunapakai dalam penentuan aktiviti pendewasaan lalat terhadap nanopartikel. Gumpalan kering dan ampaian koloid, keduanya menghasilkan kesan yang berbeza terhadap kadar kematian lalat buah, iaitu julat 25-100% dalam tempoh 12 jam pendedahan. Walau bagaimanapun, gumpalan kering menghasilkan kadar kematian yang lebih tinggi berbanding ampaian koloid. Penyelidik membuat kesimpulan bahawa sambong boleh diguna dalam penyediaan nanopartikel-yang diperkaya biosida sama ada ampaian pekat, debu atau serbuk dengan bahan jerapan yang sesuai. Sambong boleh dipertimbangkan sebagai alternatif dalam pengawalan lalat buat oriental.

 

Kata kunci:  biosida, Blumea balsamifera, nanopartikel, nanoampaian, lalat buah oriental

 

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