Malaysian Journal of Analytical Sciences Vol 22 No 1 (2018): 123 - 127

DOI: 10.17576/mjas-2018-2201-15

 

 

 

PHYTOCHEMICAL AND ANTIMICROBIAL EVALUATION OF Pithecellobium jiringa STEM BARKS EXTRACTS

 

(Penyaringan Fitokimia dan Antimikrob ke atas Ekstrak Kulit Kayu Batang Pithecellobium jiringa)

 

Zurhana Mat Hussin*, Nurul Anasuhah Osman, Aiza Harun, Shaari Daud

 

Faculty of Applied Sciences,

Universiti Teknologi MARA Pahang, 26400 Bandar Tun Abdul Razak, Jengka, Pahang, Malaysia

*Corresponding author:  zurhana_mhussin@pahang.uitm.edu.my

 

 

Received: 4 December 2016; Accepted: 1 December 2017

 

 

Abstract

Pithecellobium jiringa (P. jiringa) is economically important due to its various traditional uses in treating hypertension and diabetes (seeds), medicine (leaves) and source of dye for silk (pods). This is a pioneer study that reports its effectiveness as antimicrobial agent from stem bark of P. jiringa. The present study was carried out to detect preliminary phytochemicals and antimicrobial of solvent extracts from P. jiringa stem bark. Qualitative phytochemical analysis was performed on ethanol and ethyl acetate extracts. These extracts were investigated for their antimicrobial activity of different microorganism tests including bacteria and fungi by using agar disc diffusion technique. Tannins, flavonoids, terpenoids and saponins were the main metabolites found in phytochemical screenings. Both ethanol and ethyl acetate extracts exhibited marked antimicrobial activity against skin disease caused-microbes Staphylococcus aureus, Staphylococcus epidermidis and Trychophyton mentagrophytes with highest inhibition zone (IZ) of 23 mm at 200 mg/mL ethyl acetate extracts. The current study confirmed that the stem bark of P. jiringa has a potential source of active antimicrobial agent. Further analysis on the isolation and characterization of compound form P. jiringa stem bark, responsible for antimicrobial properties is in progress.

 

Keywords:  antifungal activity, antimicrobial activity, inhibition zone, Pithecellobium jiringa

 

Abstrak

Pithecellobium jiringa (P. jiringa) adalah penting dari segi ekonomi dengan kegunaan tradisional pelbagai dalam merawat tekanan darah tinggi dan kencing manis (biji), perubatan (daun) dan sumber pewarna untuk sutera (buah). Melalui kajian ini, melaporkan kali pertama, keberkesanannya sebagai agen antimikrob daripada kulit kayu batang P. jiringa. Kajian ini telah dijalankan untuk mengesan fitokimia awal dan antimikrob ekstrak pelarut dari kulit kayu batang P. jiringa. Analisis fitokimia kualitatif telah dilakukan ke atas ekstrak etanol dan etil asetat. Ekstrak ini telah dikaji untuk aktiviti antimikrob dengan ujian mikroorganisma yang berbeza termasuk bakteria dan kulat menggunakan teknik resapan cakera agar. Tanin, flavonoid, terpenoid dan saponin adalah metabolit utama yang diperolehi dari saringan fitokimia. Kedua-dua etanol dan etil asetat ekstrak mempamerkan tanda aktiviti antimikrob terhadap penyakit kulit yang disebabkan oleh mikrob Staphylococcus aureus, Staphylococcus epidermidis dan Trychophyton mentagrophytes dengan zon perencatan tertinggi (IZ) 23 mm pada 200 mg/mL ekstrak etil asetat. Kajian ini mengesahkan bahawa kulit kayu batang P. jiringa mempunyai sumber potensi antimikrob aktif. Analisis lanjut mengenai pengasingan dan pencirian komponen kulit kayu batang P. jiringa, bertanggungjawab untuk ciri-ciri antimikrob sedang dijalankan.

 

Kata kunci:  aktiviti antikulat, aktiviti antimikrob, zon perencatan, Pithecellobium jiringa

 

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