Malaysian Journal of Analytical Sciences Vol 22 No 1 (2018): 87 - 94

EFFECT OF EXTRACTION METHODS ON THE YIELD, FUCOSE CONTENT AND PURITY OF FUCOIDAN FROM Sargassum sp. OBTAINED FROM PULAU LANGKAWI, MALAYSIA

(Kesan Kaedah Pengekstrakan Fukoidan Terhadap Hasil, Kandungan Fukosa dan Ketulenan Fukoidan daripada Sargassum sp. dari Pulau Langkawi, Malaysia)

Bibi Marliana Baba, Wan Aida Wan Mustapha*, Lim Seng Joe

School of Chemical Sciences & Food Technology,

Faculty of Science and Technology,

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

*Corresponding author: wanaidawm@ukm.edu.my

Received: 12 April 2017; Accepted: 1 September 2017

Abstract

The objective of this study was to determine the effect of extraction methods on the yield, fucose content and purity of fucoidan that had been isolated from brown seaweed, Sargassum sp. The extraction of fucoidan was carried out based on the factorial design assigned, which involved three different parameters including two types of extraction (acid and alkaline), three different extraction temperatures (45, 65 and 85 °C) and three different extraction time (1, 3 and 5 hours). Results obtained showed that the best extraction parameter that gave the highest yield of fucoidan extract was the alkaline extraction at 65 °C for 3 hours. Alkaline extraction also produced higher purity and better quality of fucoidan compared to the fucoidan extracted by hydrochloric acidic.

Keywords: acidic extraction, alkaline extraction, fucoidan, fucose content

Abstrak

Objektif kajian adalah untuk mengkaji kesan kaedah-kaedah pengekstrakan terhadap hasil, kandungan fukosa dan ketulenan ekstrak fukoidan yang diperoleh daripada rumpai laut perang, Sargassum sp. Pengekstrakan fukoidan dilakukan berdasarkan reka bentuk eksperimen berfaktor, di mana tiga parameter yang berbeza telah ditetapkan termasuklah dua jenis pengekstrakan (berasid dan beralkali), tiga suhu pengekstrakan (45, 65 dan 85 °C) dan tiga masa pengekstrakan (1, 3 dan 5 jam). Keputusan kajian menunjukkan bahawa kaedah pengekstrakan terbaik yang memberi hasil fukoidan paling tinggi adalah melalui pengekstrakan beralkali pada suhu 65 °C selama 3 jam. Pengekstrakan beralkali juga menghasilkan ekstrak yang lebih tulen dan berkualiti berbanding dengan fukoidan yang diekstrak menggunakan asid hidroklorik.

Kata kunci: pengekstrakan berasid, pengekstrakan beralkali, fukoidan, kandungan fukosa

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