Malaysian Journal of Analytical Sciences Vol 22 No 1 (2018): 1 - 7
DOI:
https://doi.org/10.17576/mjas-2018-2201-01
SIMPLE µ-SOLID PHASE
EXTRACTION USING C18 FILM FOR THE EXTRACTION OF POLYCYCLIC AROMATIC
HYDROCARBONS IN COFFEE BEVERAGE
(Pengekstrakan µ-Fasa Pepejal yang Mudah Menggunakan
Filem C18 untuk Pengekstrakan Hidrokarbon Aromatik Polisiklik dalam
Minuman Kopi)
Saw Hong Loh1*,
Pei Ee Neoh1, Chee Tung Tai1, Sazlinda Kamaruzaman2
1School of Marine and Environmental Sciences,
Universiti Malaysia Terengganu, 21030 Kuala
Nerus, Terengganu, Malaysia
2Department of Chemistry, Faculty of Science,
Universiti
Putra Malaysia, 43400 Serdang, Selangor, Malaysia
*Corresponding author: lohsh@umt.edu.my
Received: 6
July 2017; Accepted: 15 August 2017
Abstract
Polycyclic aromatic hydrocarbons (PAHs) are
produced as by-products in the coffee roasting process. A simple and efficient
micro-solid phase extraction (µ-SPE) procedure coupled with high performance
liquid chromatography and fluorescence detection has been developed for the
simultaneous analysis of selected PAHs, namely phenanthrene (PHE) and
benzo[a]pyrene (BaP) in coffee beverage. The method offered trace detection
limits in the range of 0.003 to 0.1 µg/L and good relative recovery average in
the range of 88.3 to 95.7%. The µ-SPE procedure has been simplified by
employing C18 film as an adsorbent. The method is beneficial in
terms of rapidity, simple operation, in-expensive, consumption of micro volumes
of solvent and biodegradable agarose film as the adsorbent holder. The method
was then applied to analyse five coffee beverage samples and the results
indicated the presence of BaP in the range of 1.0 to 1.9 µg/L.
Keywords: agarose, polycyclic aromatic hydrocarbons, coffee, C18,
micro-solid phase extraction
Abstrak
Hidrokarbon
aromatik polisiklik (PAHs) dihasilkan sebagai produk sampingan dalam proses
memanggang kopi. Suatu prosedur pengekstrakan mikro-fasa pepejal (µ-SPE) yang
mudah dan cekap bergabung dengan kromatografi cecair berprestasi tinggi dan
pengesanan pendarfluor telah dibangunkan untuk analisis serentak PAHs terpilih,
iaitu fenanterena (PHE) dan benzo[a]pirena (BaP) dalam minuman kopi. Kaedah ini
menawarkan had pengesanan surih dalam julat 0.003 hingga 0.1 µg/L dan purata
perolehan semula relatif yang baik dalam julat 88.3 hingga 95.7%. Prosedur
µ-SPE ini telah diringkaskan dengan menggunakan filem C18 sebagai
penjerap. Kaedah ini berfaedah daripada aspek ianya cepat, beroperasi mudah,
tidak mahal, penggunaan isipadu pelarut yang mikro dan filem agarosa yang
terdegradasi-bio sebagai pemegang penjerap. Kaedah ini kemudian diaplikasikan
untuk menganalisa lima sampel minuman kopi dan keputusan menunjukkan kehadiran
BaP dalam julat 1.0 hingga 1.9 µg/L.
Kata
kunci:
agarosa, hidrokarbon aromatik
polisiklik, kopi, C18, pengekstrakan mikro-fasa pepejal
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