Malaysian Journal of Analytical Sciences Vol 21 No 6 (2017): 1243 - 1249

DIKETOPIPERAZINE PRODUCED BY PSYCHROPHILIC YEAST Glaciozyma antarctica PI12

(Diketopiperazin Dihasilkan oleh Yis Psikropilik Glaciozyma antarctica PI12)

Andi Rifki Rosandy¹, Muntaz Abu Bakar¹, Nurul Nadiah Abdul Rahman¹, Abdul Munir Abdul Murad²,

Azira Muhamad³, Rozida Mohd. Khalid¹*

¹School of Chemical Science and Food Technology, Faculty of Science and Technology

²School of Biosciences and Biotechnology, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

³Malaysia Genome Institute (MGI),

National Institute of Biotechnology Malaysia, Jalan Bangi, 43000 Kajang, Selangor, Malaysia

*Corresponding author: rozidakhalid@ukm.edu.my

Received: 7 June 2017; Accepted: 1 November 2017

Abstract

Four diketopiperazine derivatives; cyclo(-Pro-Val) (1), (-)-cyclo(-Pro-Tyr) (2), (-)-cyclo(-Pro-Phe) (3) and (+)-cyclo(-Pro-Leu) (4) were isolated from the ethyl acetate extract of Glaciozyma antarctica PI12, a cold-adapted yeast that belongs to family Kriegeriales. The compounds were isolated by radial chromatography and thin layer chromatography techniques and the chemical structures were elucidated by infrared (IR), ultraviolet (UV), nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry.

Keywords: Glaciozyma antarctica PI12, diketopiperazine, Kriegeriales

Abstrak

Empat terbitan diketopiperazin; siklo(-Pro-Val) (1), (-)-siklo(-Pro-Tir) (2), (-)-siklo(-Pro-Phe) (3) and (+)-siklo(-Pro-Leu) (4) yang dipencilkan daripada ektrak etil asetat Glaciozyma antarctica PI12, merupakan yis adaptasi sejuk yang terdiri dari famili Kriegeriales. Sebatian – sebatian terpencil telah diasingkan menggunakan teknik kromatografi pepejal dan kromatografi lapisan nipis dan struktur kimia dijelaskan oleh spektroskopi infra merah (IM), ultra lembayung (UL), resonans magnetik nuklear (RMN) dan spektrometri jisim.

Kata kunci: Glaciozyma antarctica PI12, diketopiperazin, Kriegeriales

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