Malaysian Journal of Analytical Sciences Vol 21 No 4 (2017): 770 - 777

DOI: https://doi.org/10.17576/mjas-2017-2104-02

 

 

 

CHITIN AND CHITOSAN EXTRACTION FROM Portunus pelagicus  

 

(Pengekstrakan Kitin dan Kitosan dari Portunus pelagicus)

 

Norhidayah M. Ahyat, Faridah Mohamad, Azrilawani Ahmad, Alyza A. Azmi*

 

School of Marine and Environmental Sciences,

Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

 

*Corresponding author: alyza.azzura@umt.edu.my

 

 

Received: 30 March 2017; Accepted: 4 June 2017

 

 

Abstract

Isolation of chitin and chitosan from natural sources are widely known as they can be used in many applications. Extraction of chitin and chitosan are carried out in exoskeleton of flower crab, Portunus pelagicus. Chemical treatments such as demineralization and deproteinzation processes are used for chitin extraction, and followed by deacetylation process of chitin, which is to produce its derivatives that known as chitosan. Characterization of chitin and chitosan has been done using Fourier Transform Infrared (FTIR) Spectroscopy, X-ray diffraction (XRD), CHNS elemental analysis and Thermogravimetric analysis (TGA). Preliminary results of FTIR vibrations patterns showed stretching of hydroxyl (O-H), amine (N-H) and carbonyl (C-O) groups indicated the presence of chitin and chitosan. XRD analysis indicates the crystalline nature of chitin and chitosan. The degree of deacetylation of chitin was characterized using CHNS elemental analyzer. The extracted chitosan will be used as reducing agent for silver nanoparticles synthesis in the future studies.

 

Keywords:  crustacean, marine invertebrates, bio-polymers, natural reductant

 

Abstrak

Pengasingan kitin dan kitosan daripada sumber semulajadi telah dikenali secara meluas kerana dapat digunakan dalam banyak aplikasi. Pengekstrakan kitin dan kitosan dari exoskeleton ketam bunga, Portunus pelagicus telah dilakukan. Rawatan kimia seperti proses demineralisasi dan proses deprotenisasi telah digunakan untuk pengekstrakan kitin dan diikuti dengan proses deasetilasi daripada ktin untuk penghasilan terbitannya yang dikenali sebagai kitosan. Pencirian kitin dan kitosan telah dilakukan dengan menggunakan Spektoskopi Inframerah Transformasi Fourier (FTIR), pembelauan Sinar X (XRD), analisis unsur CHNS dan analisis termogravimetri (TGA). Dapatan awal getaran FTIR menunjukkan regangan bagi kumpulan hidroksil (O-H), amino (N-H) dan karbonil (C-O) menunjukan kehadiran sebatian kitin dan kitosan. Analisis XRD menunjukkan sifat kristal bagi kitin dan kitosan. Pencirian darjah deasetilasi kitin telah dilakukan menggunakan penganalisis unsur CHNS. Ekstrak kitosan akan digunakan sebagai agen penurun bagi sintesis nanopartikel perak untuk kajian masa hadapan.

 

Kata kunci:  krustasea, invertebrata marin, bio-polimer, penurun semula jadi

 

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