Malaysian Journal of Analytical Sciences Vol 18 No 3 (2014): 555 - 561

 

 

 

EFFECT OF GAMMA RADIATION ON AMINO ACID BASED VESICLE CARRYING RADIOSENSITIZER

 

(Kesan Sinaran Gama ke atas Vesikel berasaskan Asid Amino yang membawa Pemeka Sinaran)

 

Nur Ratasha Alia Md. Rosli, Faizal Mohamed*, Muhammad Amir Syafiq Md. Sah, Irman Abdul Rahman

 

School of Applied Physics, Faculty of Science and Technology

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

 

*Corresponding author: faizalm@ukm.edu.my

 

 

Abstract

Vesicles has been developed and studied to be used as a medium to transport radiosensitizer in treating cancer cells by increasing its sensitivity effectively towards the radiation given during radiotherapy. This study was conducted  to investigate  the effect of gamma radiation on amino acid-based vesicle carrying radiosensitizer. Amino acid based vesicles carrying radiosensitizer were synthesized using sonication method with sodium N-lauroylsarcosinate hydrate and decanol being the primary surfactant, while hydrogen peroxide and sodium hyaluronate as the encapsulated radiosensitizer. The synthesized vesicle was then irradiated at radiation doses equilvalent to those given  during radiotherapy. Irradiated vesicle carrying radiosensitizer were then characterized using Transmission Electron Microscopy (TEM), Fourier Transform Infrared Spectroscopy (FTIR) and Polarized Light Microscope. Results obtained shows that there were no significant changes in morphology and molecular conformation of the synthesized vesicle after irradiation.  Even at higher radiation dose of 100 Gray and 200 Gray , the results remained unchanged. This indicates that the synthesized vesicle carrying radiosensitizer is morphologically and spectroscopically stable even at high radiation doses.

 

Keywords: Amino acid based Vesicle, Radiosensitizer, Radiation, Radiotherapy, Cancer

 

Abstrak

Vesikel telah dihasilkan dan dikaji sebagai medium untuk membawa pemeka sinaran dalam merawat kanser secara berkesan dengan meningkatkan kesensitifan sel kanser terhadap sinaran yang diberi ketika rawatan radioterapi. Kajian ini dijalankan untuk mengkaji kesan sinaran gama ke atas vesikel berasaskan asid amino yang membawa pemeka sinaran. Vesikel berasaskan asid amino yang membawa pemeka sinaran telah disintesis menggunakan kaedah sonikasi dengan natrium N-lauroylsarcosinat hidrat dan dekanol sebagai sufaktan utama, manakala hidrogen peroksida dan natrium hialuronat sebagai pemeka radio yang dikapsulkan.  Vesikel yang disintesis telah disinarkan dengan dos sinaran yang setara dengan dos yang diberi ketika rawatan radioterapi. Pencirian telah dilakukan ke atas vesikel membawa pemeka sinaran yang disinarkan menggunakan Mikroskopi Elektron Transmisi (TEM), Spektroskopi Inframerah Transformasi Fourier (FTIR) dan Mikroskop Cahaya Polarisasi. Hasil kajian menunjukkan bahawa tiada perubahan yang signifikan dari segi morfologi dan konformasi molekul vesikel membawa pemeka sinaran setelah disinar. Walaupun disinarkan pada dos sinaran 100 Gray dan 200 Gray, hasilnya tetap sama. Ini menunjukkan bahawa vesikel membawa pemeka sinaran yang disintesis adalah stabil dari segi morfologi dan spektroskopi terhadap sinaran walaupun dos sinaran yang tinggi telah diberikan.

 

Kata kunci: Vesikel berasaskan Asid Amino, Pemeka Sinaran, Sinaran, Radioterapi, Kanser

 

 

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