Malaysian Journal of Analytical Sciences Vol 17 No 3 (2013): 454 – 460

 

 

 

THE EFFECT OF GAMMA IRRADIATION ON THE CHEMICAL STRUCTURE AND SURFACE CHARACTERISTICS OF DIPALMITOYLPHOSPHATIDYLCHOLINE (DPPC)          

 

(Kesan Sinaran Gama ke atas Struktur Kimia dan Ciri Permukaan Dipalmitoilfosfatidilkolina (DPPC))

 

Hur Munawar Kabir Mohd1, Ainee Fatimah Ahmad1, Faizal Mohamed1, Nursaidatul Syafadillah Kamaruzaman1, Ng Wei Ling1, Poh Roe Jin1, Muhamad Samudi Yasir1, Mohd Hafez Mohd Isa3, Suria Ramli2, Shahidan Radiman1 and Irman Abdul Rahman1*

 

1School of Applied Physics,

2School of Chemical Sciences and Food Technology,

Faculty of Scince & Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.

3Industrial Chemistry Technology Program,

Faculty of Science & Technology,

Universiti Sains Islam Malaysia, Bandar Baru Nilai, 71800 Nilai, Negeri Sembilan.

 

 

*Corresponding author: irman@ukm.my

 

 

Abstract

Dipalmitoylphosphatidylcholine (DPPC) is one of the most abundant lipids in the human body and carries out important physiological functions such as liver protection and fighting infections. This study was conducted to investigate the effect of ionising radiation on the chemical structure and surface characteristic of DPPC. Synthetic DPPC was dissolved in chloroform (1mg/ml) and irradiated with Cobalt-60 (dose range of 50 – 200 Gy). The change in surface characteristics due to gamma irradiation was determined by means of monolayer compression isotherms using a Langmuir trough. From the change in the isotherm features, the threshold dose that affected the surface characteristics of the DPPC monolayer was determined to be 60 Gy. With increasing dose value, the plateau feature in the DPPC isotherm became shorter and was shifted to higher surface pressures. Analysis using High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (MS) suggested that gamma irradiation of DPPC destroyed the chemical structure of DPPC and produced two main radiolytic products, namely lisophosphatidylcholine, LPC (~495.3142 g/mol) and phosphatidic acid, PA (~718.916 g/mol) with an average percentage of LPC and PA of 23% and 74%, respectively.

 

Keywords: dipalmitoylphosphatidylcholine (DPPC), Langmuir monolayer, High Performance Liquid-Chromatography (HPLC), Mass-Spectrometer (MS), gamma radiation

 

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