The Malaysian Journal of Analytical Sciences Vol 15 No 1 (2011): 113 – 121






(Gas Chromatography x Gas Chromatography–Time-of-Flight Mass Spectrometry Analysis and Antibacterial Activity of Essential Oil from Amomum xanthophlebium)


A. Masila1, I. Aminah1, W.A. Yaacob1, I. Nazlina2


1Pusat Pengajian Sains Kimia dan Teknologi Makanan,

2Pusat Pengajian Biosains dan Bioteknologi,

Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia,

43600 Bangi, Selangor, Malaysia.


*Corresponding author:




Essential oils of fresh leaves, stem, rhizomes and whole aromatic plants of Amomum xanthophlebium (Zingiberaceae) were obtained by hydrodistillation. Percentage yields of the leaf, stem and whole plant oils were 0.0032, 0.0074 and 0.0021% whereas the rhizome oil obtained was very little. Chemical components of each oil and their percentages were determined by Gas Chromatography x Gas Chromatography–Time-of-Flight Mass Spectrometry (GCxGC-TOFMS). Analysis of A. xanthophlebium oils showed that they were dominated by terpenes. Main components in the leaves were allo-aromadendrene (3.41%), (±)-globulol (2.58%) and rosifoliol (2.55%); stem, α-terpineol (4.25%), rosifoliol (2.41%) and bingpian (2.27%); rhizomes, viridiflorol (5.72%), (±)-globulol (5.23%) and α-cadinol (4.81%); whole plants, eucalyptol (4.11%), l-α-terpineol (2.88%) and rosifoliol (2.82%). The stem oil of A. xanthophlebium showed antibacterial activity against Gram-negative Escherichia coli and Gram-positive methicillin-resistant Staphylococcus aureus (MRSA) at the minimum inhibitory concentration of 80 mg/ml.


Keywords: Amomum xanthophlebium, essential oil, GCxGCTOFMS



1.       Habsah, M., Ali, A.M., Lajis, N.H., Sukari, M.A., Yap, Y.H., Kikuzaki, H., Nakatani, N. 2005. Antitumor promoting and cytotoxic constituents of Etlingera elatior. Malaysian Journal of Medical Sciences. 12(1): 6-12.

2.       Zakaria, M.B. & Ibrahim, H. 1986. Phytochemical screening of some Malaysian species of Zingiberaceae. Malaysian Journal of Science 8: 125-128.

3.       Takano, A., Hernawati & Tamin, R. 2001. Notes on Globba Linnaeus in Sumatra, W. Malaysia & Borneo: a new record of Globba brachyanthera var. rubra in Sumatra. Folia Malaysiana 2(1): 25-34.

4.       Ibrahim, H. & Rahman, A.A. 1988. Several ginger plants (Zingiberaceae) of potential value. Proceeding of the Seminar on Malaysian Traditional Medicine, hlm. 159-161. Kuala Lumpur: IPT, University Malaya.

5.       Mathew, J., Subulal, B., George, V., Dan, M. & Shiburaj, S. 2006. Chemical composition and antimicrobial    activity of the leaf oil of Amomum cannicarpum (Wight) Bentham ex Baker. Journal of Essential Oil Research 18(1): 35-37.

6.       Sabulal, B., Dan, M., Pradeep, N.S., Valsamma, R.K. & George V. 2006. Composition and antimicrobial activity of essential oil from the fruits of Amomum cannicarpum.  Acta Pharmaceutica 56(4): 473–480.

7.       Sabulal, B., Dan, M., John J.A., Kurup, R., Pradeep, N.S., Valsamma, R.K. & George, V. 2006. Caryophyllene-rich rhizome oil of Zingiber nimmonii from South India: chemical characterization and antimicrobial activity. Phytochemistry 67(22): 2469-2473.

8.       Hazarika, A.K. & Nath, S.C. 1995. Methyl chavicol-the major component of the rhizome oil of Amomum linguiforme Benth. Journal of Essential Oil Research 7(3): 325-326.

9.       Dallüge, J., van Stee, L.L.P., Xu, X., Williams, J., Beens, J., Vreuls, R.J.J. & Brinkman, U.A.T. 2002. Unravelling the composition of very complex samples by comprehensive gas chromatography coupled to time-of-flight mass spectrometry. cigarette smoke. Journal of Chromatography, A 974(1-2): 169-184.

10.    Hussain, A.I., Anwar, F., Hussain Sherazi, S.T. & Przybylski, R. 2008. Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chemistry 108(3): 986-995.

11.    Yaacob, K.B. 1988. Antimicrobial properties of essential oils. Proceeding of the Seminar on Malaysian Traditional Medicine, hlm. 125-129. Kuala Lumpur: IPT, University Malaya.




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