The Malaysian Journal of Analytical Sciences Vol 15 No 2 (2011): 240 –
251
DEVELOPMENT AND VALIDATION OF RP-HPLC-UV/Vis METHOD
FOR DETERMINATION OF PHENOLIC COMPOUNDS IN SEVERAL
PERSONAL CARE PRODUCTS
(Pembangunan dan Validasi Kaedah
Fasa Berbalik-HPLC-UV/Vis
untuk Penentuan Sebatian
Fenolik dalam beberapa Produk Penjagaan Diri)
Mohammed
Akkbik, Zaini Bin Assim*, Fasihuddin B.
Ahmad
Department of Chemistry,
Faculty of Resource Science and Technology, Universiti Malaysia
Sarawak,
94300 Kota Samarahan, Sarawak, Malaysia
*Corresponding author: zaini@frst.unimas.my
Abstract
A HPLC method
with ultraviolet-visible spectrophotometry detection has been optimized and
validated for the simultaneous determination of phenolic compounds, such as butylated hydroxyanisole (BHA) and butylated
hydroxytoluene (BHT) as antioxidants, and octyl methylcinnamate (OMC) as
UVB-filter in several personal care products. The dynamic range was between 1
to 250 mg/L with relative standard deviation less than 0.25%, (n=4). Limit of
detection for BHA, BHT and OMC were 0.196, 0.170 and 0.478 mg/L, respectively. While limit of quantification for BHA, BHT
and OMC were 0.593, 0.515 and 1.448 mg/L, respectively. The recovery for
BHA, BHT and OMC ranged from 92.1-105.9%, 83.2-108.9% and 87.3-103.7%,
respectively. The concentration ranges of BHA, BHT and OMC in 12 commercial
personal care samples were 0.13-4.85, 0.16-2.30 and 0.12-65.5 mg/g,
respectively. The concentrations of phenolic compounds in these personal care
samples were below than maximum allowable concentration in personal care
formulation i.e 0.0004 – 10 mg/g, 0.002 – 5 mg/g and up to 100 mg/g for BHA,
BHT and OMC, respectively.
Keywords: Phenolic
compounds, personal care products, RP-HPLC-UV/Vis, optimization and validation method.
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