The Malaysian Journal of Analytical Sciences Vol 15 No 2 (2011): 240 – 251

 

 

 

DEVELOPMENT AND VALIDATION OF RP-HPLC-UV/Vis METHOD

FOR DETERMINATION OF PHENOLIC COMPOUNDS IN SEVERAL PERSONAL CARE PRODUCTS

 

(Pembangunan dan Validasi  Kaedah Fasa Berbalik-HPLC-UV/Vis

untuk Penentuan Sebatian Fenolik dalam beberapa Produk Penjagaan Diri)

 

Mohammed Akkbik, Zaini Bin Assim*,  Fasihuddin B. Ahmad

 

Department of Chemistry,

Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 

94300 Kota Samarahan, Sarawak, Malaysia

 

*Corresponding author: zaini@frst.unimas.my

 

 

Abstract

A HPLC method with ultraviolet-visible spectrophotometry detection has been optimized and validated for the simultaneous determination of phenolic compounds, such as butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) as antioxidants, and octyl methylcinnamate (OMC) as UVB-filter in several personal care products. The dynamic range was between 1 to 250 mg/L with relative standard deviation less than 0.25%, (n=4). Limit of detection for BHA, BHT and OMC were 0.196, 0.170 and 0.478 mg/L, respectively. While limit of quantification for BHA, BHT and OMC were 0.593, 0.515 and 1.448 mg/L, respectively. The recovery for BHA, BHT and OMC ranged from 92.1-105.9%, 83.2-108.9% and 87.3-103.7%, respectively. The concentration ranges of BHA, BHT and OMC in 12 commercial personal care samples were 0.13-4.85, 0.16-2.30 and 0.12-65.5 mg/g, respectively. The concentrations of phenolic compounds in these personal care samples were below than maximum allowable concentration in personal care formulation i.e 0.0004 – 10 mg/g, 0.002 – 5 mg/g and up to 100 mg/g for BHA, BHT and OMC, respectively.

 

Keywords: Phenolic compounds, personal care products, RP-HPLC-UV/Vis, optimization and validation method.

 

References

1.     Tsai, T.F., Lee, M.R. (2008). Determination of antioxidants and preservatives in cosmetics by SPME combined with GC–MS. Chromatographia, 67: 425-431.

2.     Chawla, H.M., Mrig, S. (2009). Simultaneous quantitative estimation of oxybenzone and 2-ethylhexyl-4-methoxycinnamate in sunscreen formulations by second order derivative spectrophotometry. Journal of Analytical Chemistry, 64(6): 585–592.

3.     Yang, T.J., Tsai, F.J., Chen, C.Y., Yang, T.C.C. & Lee, M.R. (2010). Determination of additives in cosmetics by supercritical fluid extraction on-line headspace solid-phase microextraction combined with gas chromatography–mass spectrometry. Journal of Analytical Chemistry for Anti-Counterfeiting Trade Agreement, 668:188-194.

4.     Capitan-Vallvey, L. F., Valencia, M. C., Nicolas, E. A. (2002). Flow-through sensor for determination of butylated hydroxytoluene in cosmetics. Analytical Letters, 35(1): 65-81.

5.     Capitan-Vallvey, L. F., Valencia, M. C., Nicolas, E. A. (2004). Solid-phase ultraviolet absorbance spectrophotometric multisensor for the simultaneous determination of butylated hydroxytoluene and co-existing antioxidants. Journal of Analytical Chemistry for Anti-Counterfeiting Trade Agreement, 503: 179-186.

6.     Porat, Y., Abramowitz, A., Gazit, E. (2006). Inhibition of amyloid fibril formation by polyphenols: Structural similarity and aromatic interactions as a common inhibition mechanism. Chemical Biology and Drug Design, 67: 27–37.

7.     Stockmann, H., Schwarz, K., Huynh, T. (2000). The influence of various emulsifiers on the partitioning and antioxidant activity of hydroxybenzoic acids and their derivatives in oil-in-water emulsions. Journal of Surfactants and Detergents, 77(5): 535-542.

8.     Wang, S.P., Chen, W.J. (2000). Determination of p-aminobenzoates and cinnamate in cosmetic matrix by supercritical fluid extraction and micellar electrokinetic capillary chromatography. Journal of Analytical Chemistry for Anti-Counterfeiting Trade Agreement, 416: 157-167.

9.     Chisvert, A., Salvador, A., Pascual-Marti, M.C. (2001). Simultaneous determination of oxybenzone and 2-ethylhexyl 4-methoxycinnamate in sunscreen formulations by flow injection-isodifferential derivative ultraviolet spectrometry. Journal of Analytical Chemistry for Anti-Counterfeiting Trade Agreement, 428: 183-190.

10.  Dutra, E.A., Oliveira, D.A.G.D., Kedor-Hackmann, E.R.M., Santoro, M.L.R.M. (2004). Determination of sun protection factor (SPF) of sunscreens by ultraviolet spectrophotometry. Brazilian Journal of Pharmaceutical Sciences, 40(3): 381-385.

11.  Salvador, A., Chisvert, A. (2005). An environmentally friendly (green) reversed-phase liquid chromatography method for UV filters determination in cosmetics. Journal of Analytical Chemistry for Anti-Counterfeiting Trade Agreement, 537: 15-24.

12.  Venkatesh, G., Majid, M. I. A., Ramanathan, S., Mansor, S. M., Nair, N. K., Croft, S. L., Navaratnam, V. (2008). Optimization and validation of RP-HPLC-UV method with solid-phase extraction for determination of buparvaquone in human and rabbit plasma: application to pharmacokinetic study. Biomedical Chromatography, 22: 535–541.

13.  Lee, M. R., Lin, C. Y., Li, Z. G., Tsai, T. F. (2006). Simultaneous analysis of antioxidants and preservatives in cosmetics by supercritical fluid extraction combined with liquid chromatography–mass spectrometry. Journal of Chromatography A, 1120: 244-251.

14.    Saad, B., Sing, Y.Y., Nawi, M. A., Hashim, N., Ali, A. M., Saleh, M. I., Ahmad, K. (2007). Determination of synthetic phenolic antioxidants in food items using reversed-phase HPLC. Food Chemistry, 105: 389–394.

15.  Song, D., Wang, J. 2003. Modified resolution factor for asymmetrical peaks in chromatographic separation. Journal of Pharmaceutical and Biomedical Analysis, 32: 1105-1112.

16.  Orsi, D.D., Giannini, G., Gagliardi, L., Porra, R., Berri, S., Bolasco, A., Carpani, I., Tonelli, D. (2006). Simple extraction and HPLC determination of UV-A and UV-B filters in Sunscreen. Chromatographia, 64: 9-10.

17.  Fang, F., Jing-Ming, L., Qiu-Hong, P., Wei-Dong, H. (2007). Determination of red wine flavonoids by HPLC and effect of aging. Food Chemistry, 101: 428–433.

18.  Neungnapa, R., Jia, Z., Xuewu, D., Bao, Y., Jianrong, L., Yueming, J. (2008). Effects of various temperatures and pH values on the extraction yield of phenolics from litchi fruit pericarp tissue and the antioxidant activity of the extracted anthocyanins. International Journal of Molecular Sciences, 21:105-116.

19.  Dondi, D., Albini, A., Serpone, N. (2006). Interactions between different solar UVB/UVA filters contained in commercial suncreams and consequent loss of UV protection. The Royal Society of Chemistry and Owner Societies, 5: 835-843.

20.  Perrin, C., Meyer, L. (2003). Simultaneous determination of ascorbyl palmitate and nine phenolic antioxidants in vegetable oils and edible fats by HPLC. Journal of the American Oils Chemist’s Society, 80 (2): 115 -118.

21.  Tsuji, S., Nakano, M., Terada, H., Tamura, Y., Tonogal, Y. (2005). Determination and confirmation of five phenolic antioxidants in foods by LC/MS and GC/MS. Japanese Society of Food Hygienically, 46(3): 63-71.

22.  Campos, G.C.M.D., Toledo, M.C.F. (2000). Determination of BHA, BHT and TBHQ in fats and oils by high performance liquid chromatography. Brazilian Journal of Food Technology, 3: 65-71.

23.  Perrin, C., Meyer, L. (2002). Quantification of synthetic phenolic antioxidants in dry foods by reversed-phase HPLC with photodiode array detection. Food Chemistry, 77:  93–100.

24.  Mazonakis, N.E., Karathanassi, P.H., Panagiotopoulos, D.P., Hamosfakidi, P.G., Melissos, D.A. (2002). Cleaning validation in the toiletries industry. Journal of Analytical Chemistry for Anti-Counterfeiting Trade Agreement,  467: 261-266.

25.  Chisvert, A., Pascual-Marti, M.C., Salvador, A. (2001b). Determination of UV-filters in sunscreens by HPLC. Journal of Analytical Chemistry, 369: 638–641.

26.  Tsai, T. F., Lee, M. R. (2008). Determination of antioxidants and preservatives in cosmetics by SPME combined with GC-MS. Chromatographia, 67: 425-431.

27.  Garcia-Jimenez, J.F., Valencia, M.C., Capitan-Vallvey, L.F. (2007). Simultaneous determination of antioxidants, preservatives and sweetener additives in food and cosmetics by flow injection analysis coupled to a monolithic column. Journal of Analytical Chemistry for Anti-Counterfeiting Trade Agreement, 594: 226-233.

28.  Gao, T., Bedell, A. (2001). Ultraviolet damage on natural gray hair and its photoprotection. Journal of Cosmetic Science, 52: 103-118.

29.  Fent, K., Kunz, P.Y., Zenker, A., Rapp, M. (2009). A tentative environmental risk assessment of the UV-filters 3-(4-methylbenzylidene-camphor) 2-ethyl-hexyl-4-trimethoxycinnamate, benzophenone-3, benzophenone-4 and 3-benzylidene camphor. Marine Environmental Research, 10: 1016-1018.

 

 

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