Malays. J. Anal. Sci. Volume 29 Number 2 (2025): 1434

 

Research Article

 

Antioxidant activity and cytotoxicity of active isolates from Chloranthus erectus leaves against T47D and 4T1 breast cancer cell lines

 

Dinda Putri Faristi1, Haryoto1*, Noor Hidayah Pungot3,4, and Agustono Wibowo2,4

 

1Faculty of Pharmacy, Muhammadiyah Surakarta University, Sukoharjo, 57169 Central Java, Indonesia

2Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM) Pahang Branch, Jengka Campus, 26400 Bandar Tun Abdul Razak Jengka, Pahang, Malaysia

3Faculty of Applied Sciences, Universiti Teknologi MARA (UiTM), 40450 Shah Alam, Selangor, Malaysia

4Organic Synthesis Laboratory, Institute of Science, Universiti Teknologi MARA (UiTM), 42300 Bandar Puncak Alam, Selangor, Malaysia

 

*Corresponding author: har254@ums.ac.id

 

Received: 9 December 2024; Revised: 5 March 2025; Accepted: 13 March 2025; Published: 21 April 2025

 

Abstract

Breast cancer is one of the most common types of cancer in women and ranks fifth leading cause of cancer death globally. One of the contributing factors to various diseases such as cancer, inflammation, atherosclerosis, and premature aging is the presence of reactive oxygen species, including hydrogen peroxide, superoxide, hydroxyl radicals, and other radical compounds. This study aimed to evaluate the antioxidant properties and cytotoxic activity of extracts derived from the leaves of Chloranthus erectus. The antioxidant properties were assessed using DPPH, ABTS, and FRAP assays, while the cytotoxic activity was tested against T47D and 4T1 breast cancer cell lines using the MTT assay method. The study discovered that the antioxidant activity of isolates from C. erectus exhibited moderate effects, with IC50 values ranging from 108 - 131 µg/ml. In cytotoxicity tests, Isolate 3 demonstrated an IC50 value of 45.38 ± 23.40 µg/ml against 4T1 cells, while Isolate 2 had an IC50 of 94.42 ± 5.90 µg/ml against T47D cells. These results suggest that both isolates exhibit moderate to strong anticancer activity. Additionally, phytochemical screening of Isolates 1-3, revealed the presence of alkaloids, flavonoids, steroids, polyphenols, and saponins.

 

Keywords: Chloranthus erectus, DPPH, FRAP, ABTS, T47D

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