Malays. J. Anal. Sci. Volume 29 Number 1 (2025): 1289

 

Research Article

 

Analysis of antioxidant and antimicrobial potentials in Hemerocallis fulva flower extract via thin-layer chromatography-direct bioautography (TLC-DB)

 

Nurul Syazni Mohd Zin1, Fazilah Ariffin1,3, Ahmad Nazif Aziz1,2, Asnuzilawati Asari1,2, Maulidiani Maulidiani1,2 and Nurul Huda Abdul Wahab1,2*

 

1Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Malaysia

2Advanced Nano Materials (ANoMa) Research Group, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Malaysia

3Biological Security and Sustainability (Bioses) Research Interest Group, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Malaysia

 

*Corresponding author: nhuda@umt.edu.my

 

Received: 25 August 2024; Revised: 3 December 2024; Accepted: 5 December 2024; Published: 1 February 2025

 

Abstract

Hemerocallis fulva (daylily) has been traditionally used to treat a variety of ailments. This study aimed to identify the potential secondary metabolites present in Malaysian H. fulva (Asphodelaceae) and assess their potential antioxidant and antimicrobial activities by using thin layer chromatography-direct bioautography (TLC-DB). The dried flowers of H. fulva were extracted by using hexane, ethyl acetate, and methanol. Both qualitative and quantitative phytochemical analyses were performed on the crude extracts. The results indicated that alkaloids, flavonoids, quinones, proteins, and steroids were predominant in all samples extracted with the three solvents. In the quantitative analysis, methanol extract of the flowers had the highest flavonoid content (290.25 ± 0.02 mg QE/g), while ethyl acetate extract had the highest phenolic content (66.82 ± 0.06 mg GAE/g) as compared to other extracts. Additionally, the TLC-DB assay demonstrated that each extract of H. fulva flowers possesses significant antioxidant and antimicrobial activities. These findings provide valuable preliminary data that may contribute to the further development of this plant as a potential source of herbal medicine.

 

Keywords: Hemerocallis fulva, antioxidant, antimicrobial, thin-layer chromatography direct bioautography, phenolic

 

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