Malaysian Journal of Analytical Sciences, Vol 28 No 5 (2024): 1115 - 1127

 

FABRICATION AND CHARACTERIZATION OF POLY(3-HYDROXYBUTYRATE)-BLEND SCAFFOLDS

 

(Pembuatan dan Pencirian Rangkaian Campuran Poli (3-Hidroksibutrirat))

 

Nallusamy NithisKanna1, Afiqah Najwa2, Anis Suraya Rosli2, Nabilah Hasbullah2, Mas Mohammed2, Wan M. Khairul2, Amirul Al-Ashraf3,4,5, and Sevakumaran Vigneswari1*

 

1Institute of Climate Adaptation and Marine Biotechnology (ICAMB), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

2Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia

3School of Biological Science, Universiti Sains Malaysia, 11800 Minden, Pulau Pinang, Malaysia

4Centre for Chemical Biology, Universiti Sains Malaysia, 11900 Bayan Lepas, Penang, Malaysia

5Malaysian Institute of Pharmaceuticals and Nutraceuticals, National Institutes of Biotechnology Malaysia, 11700 Gelugor, Penang, Malaysia

 

*Corresponding author: vicky@umt.edu.my

 

 

Received: 9 March 2024; Accepted: 17 July 2024; Published:  27 October 2024

 

 

Abstract

The development of advanced wound dressings heavily relies on biomaterials, particularly biopolymeric scaffolds. These scaffolds are ideal candidates for dressings due to their good permeability, excellent biocompatibility, biodegradation, antimicrobial properties, and ability to provide a moist environment for wound repair. These features overcome the shortcomings of traditional dressings. In the pursuit of advanced wound dressing materials, this study fabricates distinct scaffolds comprising poly(3-hydroxybutyrate) P(3HB) with chitosan, polyvinyl alcohol (PVA), carboxymethyl cellulose (CMC), and silver sulfadiazine (SSD). The scaffolds are characterised using scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC) revealing their unique properties. SEM analysis provides insights into the microstructure, showing the presence of open surface pores and connected bulk pores that facilitate homogeneous cell distribution and seeding, promoting tissue regeneration. FTIR analysis of the P(3HB) blended film reveals characteristic bands, including carbonyl groups, hydroxyl groups, alkyl groups, and amine groups. TGA and DSC revealed stage-dependent decomposition and varied thermal behaviour depending on the scaffold composition, with insights into moisture retention and potential decomposition of specific components. Our findings shed light on the structural and chemical characteristics of these scaffolds, enhancing our understanding of their suitability for wound dressing applications. The information gained from this study contributes to the development of more effective and biocompatible wound dressing materials, potentially revolutionising the field of wound care.

 

Keywords: poly(3-hydroxybutyrate), chitosan, polyvinyl alcohol, carboxymethyl cellulose, thermogravimetric analysis

 

 

Abstrak

Pembangunan biobahan berasaskan polimer sangat bergantung kepada sesuatu biobahan tersebut. Biobahan ini adalah calon yang sesuai untuk pembalut kerana kebolehtelapannya yang baik, biokompatibiliti yang sangat baik, biodegradasi, sifat antimikrob dan keupayaan untuk menyediakan persekitaran yang lembap untuk pembaikan luka bagi mengatasi kekakangan pembalut luka tradisional. Dalam usaha membangunkan perencah yang menepati kriteria sebagai perencah pembalut luka yang baik, kajian ini telah ditumpukan untuk menghasilkan dua perancah berbeza yang terdiri daripada poli(3-hidroksibutirat) (P3HB) dengan kitosan, polivinil alkohol (PVA), karboksimetil selulosa (CMC), dan sulfadiazin perak (SSD). Perancah yang dihasilkan ini dicirikan menggunakan analisis SEM dan FTIR, mencirikan sifatnya. Analisis SEM menunjukkan struktur mikro, menunjukkan kehadiran liang permukaan yang memudahkan pengedaran dan pembenihan sel dan sekaligus menggalakkan penjanaan semula tisu. Analisis FTIR bagi filem campuran P(3HB) mendedahkan kumpulan karbonil, kumpulan hidroksil, kumpulan alkil dan kumpulan amina. Penemuan kami memberi penerangan tentang ciri-ciri struktur dan kimia perencah ini, meningkatkan pemahaman kami tentang kesesuaian mereka untuk aplikasi pembalut luka. Maklumat yang diperoleh daripada kajian ini menyumbang kepada pembangunan bahan pembalut luka yang lebih berkesan dan biokompatibel, yang berpotensi merevolusikan bidang penjagaan luka.

Kata kunci: poli(3-hidroksibutirat), kitosan, polivinil alkohol, karboksimetil selulosa, analisis termogravimetri

 

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