Malaysian Journal of Analytical Sciences, Vol 28 No 4 (2024): 801 - 811

 

MULTIPLE REACTION MONITORING (MRM) OF PORCINE-SPECIFIC PEPTIDE MARKERS FROM LACTATE DEHYDROGENASE AND SERUM ALBUMIN IN PROCESSED MEAT FOR HALAL AUTHENTICATION

 

(Pemantauan Tindak Balas Pelbagai (MRM) Penanda Peptida Khusus bagi Khinzir dari Lactate Dehydrogenase dan Serum Albumin dalam Daging yang Diproses

untuk Pengesahan Halal)

 

Siti Aimi Sarah Zainal Abidin1,2, Awis Qurni Sazili3,4, Wan Nur Faradalila3, Ismail Amin5,

and Saiful Anuar Karsani6,7*

 

1Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia

2Malaysia Institute of Transport, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia

3Halal Products Research Institute, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

4 Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

5 Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences,

Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

6 Institute of Biological Sciences, Faculty of Science,Universiti Malaya, 50603 Kuala Lumpur, Malaysia

 7 University of Malaya Center for Proteomics Research, Universiti Malaya, 50603 Kuala Lumpur, Malaysia

 

*Corresponding author: saiful72@um.edu.my

 

 

Received: 15 September 2023; Accepted: 11 June 2024; Published:  27 August 2024

 

Abstract

Multiple Reaction Monitoring (MRM) of four peptides is presented to detect the presence of pork in meat products using a triple quadrupole system (LC-QQQ-MS). Commercial samples were digested using the tryptic digestion method. MRM acquisition was optimized using Skyline software. Then, the presence of peptides was tested and verified on processed meat products, each with a different type of processing. The result reveals that peptides EVTEFAK (m/z = 450.2873), LVVITAGAR (m/z = 412.2134), FVIER (m/z = 388.7369), and TVLGNFAAFVQK (m/z = 647.8613) were consistently detected in the processed meat and displayed porcine-specific properties. Peptide LVVITAGAR, the most intense peptide, was eluted at 7 min while FVIEIR, as the second highest peak, was eluted at 9.5 min. On the other hand, peptide EVTEFAK was eluted at 4.5 min, and TVLGNFAAFVQK, a peptide with the lowest intensity, appeared between 12.5 to 13 min. Specifically, one peptide is derived from lactate dehydrogenase, and three from serum albumin protein. It demonstrated that porcine-specific peptides could be simultaneously targeted by the MRM method with the help of Skyline software that helps optimize the analytes' specific precursor ion, product ion, and fragmentation behaviour under collision energy. The present result reveals that MRM will enable rapid halal detection of contaminants occurring during the manufacturing or supply chain and ensure food integrity is preserved along the food supply chain.

 

Keywords: triple quadrupole, halal supply chain, peptide marker, species authentication

 

Abstrak

Pemantauan tindak balas berganda (MRM) empat peptida untuk mengesan kehadiran daging khinzir dalam produk daging menggunakan sistem caturkutub ganda tiga (LC-QQQ-MS) adalah dilaporkan. Sampel komersil dicerna dengan kaedah penghadaman tryptic. Parameter pemerolehan MRM telah dioptimumkan menggunakan perisian Skyline. Seterusnya, kehadiran peptida telah diuji dan disahkan pada produk daging yang diproses dimana setiap satunya melalui jenis pemprosesan yang berbeza. Keputusan menunjukkan bahawa peptida EVTEFAK (m/z = 450.2873), LVVITAGAR (m/z = 412.2134), FVIER (m/z = 388.7369) dan TVLGNFAAFVQK (m/z = 647.8613) dikesan secara konsisten dan mempunyai sifat khusus daging khinzir. Peptida EVTEFAK, LVVITAGAR, FVIER dan TVLGNFAAFVQK dikesan secara konsisten dalam daging yang diproses dan menunjukkan sifat khusus khinzir. Satu peptida diperoleh khusus daripada laktat dehidrogenase dan tiga daripadanya adalah protein serum albumin. Ia menunjukkan bahawa peptida khusus untuk mengesan khinzir boleh disasarkan secara serentak oleh kaedah MRM dengan syarat pengetahuan tentang ion prekursor khusus analit, ion produk dan tingkah laku pemecahan di bawah tenaga perlanggaran dioptimumkan. Keputusan kajian menunjukkan bahawa MRM akan membolehkan pengesanan cepat bahan halal tercemar yang berlaku semasa proses pembuatan atau rantaian bekalan dan memastikan integriti makanan terpelihara di sepanjang rantaian bekalan makanan.

 

Kata kunci: caturkutub ganda tiga, rantaian bekalan halal, penanda peptida, pengesahan spesies

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