Malaysian Journal of Analytical Sciences, Vol 28 No 4 (2024): 768 - 780

 

PHYTOCHEMICAL PRODUCTION ENHANCEMENT ON IN VITRO CULTURE OF Clinacathus nutans

 

(Peningkatan Pengeluaran Fitokimia Pada Kultur In Vitro Clinacathus nutans)

 

Saiyidah Nafisah Hashim1, Siti Mazleena Mohamed1, Norrizah Jaafar Sidik2* Mohd Farid Mohd Khotob2,

and Tay Chia Chay2

 

1Faculty of Applied Sciences, UiTM Perak Branch, Tapah Campus, 35400 Tapah Road, Perak

2Faculty of Applied Sciences, UiTM Shah Alam, 40450 Shah Alam Selangor Darul Ehsan

 

 

*Corresponding author: norri536@uitm.edu.my

 

 

Received: 17 September 2023; Accepted: 2 June 2024; Published:  27 August 2024

 

 

Abstract

This study assessed the effects of 6-Benzylaminopurine and wood vinegar on in vitro shoot multiplication, and as elicitors in enhancing the phytochemical content of Clinacanthus nutans extract. A nodal explant of C. nutans was cultured in vitro with single or combination treatments in MS medium supplemented with 6-Benzylaminopurine (BAP) or wood vinegar (WV). The growth performance of regenerated shoots was documented after eight weeks of culture. The total phenolic content, total flavonoid content, and antioxidant activities of the leaf extracts were also studied. The results demonstrated that all single treatments with BAP successfully regenerated and multiplied shoots and leaves. While in combination treatments, the data revealed that 2 mg/L BAP with 1% WV (B2WV1) medium treatment led to the highest number of shoots and leaves per explant and the highest total phenolic content and antioxidant activities in the leaf extract. This concludes that combining wood vinegar and BAP treatments in the culture medium caused significant shoot and leaf growth and enhanced the production of leaf’s secondary metabolites. These findings highlight the potential of 6-Benzylaminopurine and wood vinegar as elicitors to enhance the production of secondary metabolites in Clinacanthus nutans, providing valuable insights for further research in plant biotechnology.

 

Keywords: Clinacanthus nutans, 6-Benzylaminopurine, wood vinegar, in vitro, elicitor, phytochemicals

 

Abstrak

Kajian ini menilai potensi 6-Benzilaminopurin dan cuka kayu untuk penambahan jumlah pucuk in vitro dan sebagai elisitor dalam meningkatkan kandungan fitokimia ekstrak Clinacanthus nutans. Eksplan nod C. nutans dibiakkan secara in vitro dengan rawatan tunggal atau gabungan dalam medium MS ditambah dengan 6-Benzilaminopurin (BAP) atau cuka kayu (WV). Prestasi pertumbuhan pucuk yang dijana semula telah didokumenkan selepas lapan minggu kultur. Jumlah kandungan fenolik, jumlah kandungan flavonoid, dan aktiviti antioksidan ekstrak daun turut dikaji. Keputusan menunjukkan bahawa semua rawatan tunggal dengan BAP berjaya menjana semula dan membiak pucuk dan daun. Manakala rawatan gabungan menunjukkan bahawa 2 mg/L BAP dengan 1% WV (B2WV1) membawa kepada bilangan pucuk dan daun tertinggi bagi setiap eksplan, serta jumlah kandungan fenolik dan aktiviti antioksidan tertinggi dalam daun. ekstrak. Ini menyimpulkan bahawa menggabungkan cuka kayu dan rawatan BAP dalam medium kultur menyebabkan pertumbuhan pucuk dan daun yang ketara serta meningkatkan pengeluaran metabolit sekunder daun. Penemuan ini menyerlahkan potensi 6-Benzilaminopurin dan cuka kayu sebagai elisitor untuk meningkatkan pengeluaran metabolit sekunder dalam Clinacanthus nutans, memberikan pandangan berharga untuk penyelidikan lanjut dalam bioteknologi tumbuhan.

Kata kunci: Clinacanthus nutans, 6-Benzilaminopurin, cuka kayu, in vitro, elisitor, fitokimia

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