Malaysian Journal of Analytical Sciences, Vol 28 No 2 (2024): 412 - 422

DETERMINATION OF CELECOXIB IN HUMAN PLASMA BY EFFERVESCENCE-ASSISTED DISPERSIVE LIQUID–LIQUID MICROEXTRACTION AND HPLC/UV 

 
(Penentuan Celecoxib dalam Plasma Manusia dengan Bantuan Buih Berbuak Pengekstrakan Mikro Penyebaran Cecair-ke-Cecair dan HPLC/UV)
 

Zahra Saharkhiz1*, Mohammad Reza Hadjmohammadi1, and Pourya Biparva2

 

1Department of Analytical Chemistry, Faculty of Chemistry, University of Mazandaran, NirooHavayii Boulevard, 47416-95447 Babolsar, Iran

2Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, India

 

*Corresponding author: z_saharkhiz@yahoo.com

 

 

Received: 15 November 2023; Accepted: 29 February 2024; Published:  29 April 2024

 

 

Abstract

In this study, effervescence-assisted dispersive liquid–liquid microextraction was utilized for rapid, simple, and inexpensive microextraction of celecoxib followed by HPLC/UV. In the proposed method, carbon dioxide microbubbles were produced by the reaction between oxalic acid and sodium bicarbonate. Various factors such as type and volume of extraction solvent, pH of the sample solution, content of the acidic and basic promoters, and type and volume of auxiliary solvent were optimized. Under optimum conditions, 60 μL chloroform (as an extraction solvent) was added to 2 mL methanol and 0.4g oxalic acid. The mixture was added to the test tube containing 0.8g sodium bicarbonate and 10 mL of sample solution (pH 6). The presented method offered wide linear dynamic range of 0.5-1000 μg L-1 with satisfactory extraction recovery of 94.4%, high pre-concentration factor of 206, and normal RSD% (2.15, n=3). Finally, the proposed method was successfully applied for the analysis of celecoxib in plasma samples.

 

Keywords: effervescence-assisted liquid-microextraction, celecoxib, plasma, acid-base reaction, carbon dioxide microbubbles

 

Abstrak

Dalam kajian ini, pengekstrakan mikro cecair-ke-cecair yang dibantu buih berbuak telah digunakan untuk pengekstrakan celecoxib yang cepat, mudah, dan murah diikuti oleh HPLC/UV. Dalam kaedah yang dicadangkan, buih mikro karbon dioksida dihasilkan oleh tindak balas antara asid oksalik dan natrium bikarbonat. Pelbagai faktor seperti jenis dan isipadu pelarut pengekstrakan, pH larutan sampel, kandungan penggalak asid dan bes, dan jenis dan isipadu pelarut bantu telah dioptimumkan. Di bawah keadaan optimum, 60 μL kloroform (sebagai pelarut pengekstrakan) ditambahkan ke dalam 2 mL metanol dan 0.4g asid oksalik. Campuran tersebut ditambahkan ke dalam tabung uji yang mengandungi 0.8g natrium bikarbonat dan 10 mL larutan sampel (pH 6). Kaedah yang dikemukakan menawarkan julat dinamik linear yang luas dari 0.5-1000 μg L-1 dengan pulihkan pengekstrakan yang memuaskan sebanyak 94.4%, faktor pra-pemekatan tinggi sebanyak 206, dan RSD% normal (2.15, n=3). Akhirnya, kaedah yang dicadangkan telah berjaya digunakan untuk analisis celecoxib dalam sampel plasma.

 

Kata kunci: pengekstrakan cecair-mikro yang dibantu buih berbuak, celecoxib, plasma, tindak balas asid-bes, buih mikro karbon dioksida

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