Malaysian Journal of Analytical Sciences, Vol 28 No 1 (2024): 116 - 126

 

EXTRACTION OF β-KERATIN FROM POULTRY FEATHER WASTE USING SODIUM METABISULFITE AND SODIUM DODECYL SULFATE

 

(Pengekstrakan β-Keratin dari Sisa Bulu Ayam Menggunakan Natrium Metabisulfit dan Natrium Dodesil Sulfat)

 

Soerya Dewi Marliyana*, Theresia Inna Sekar Tungga Dewi, and Triana Kusumaningsih

 

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta 57126

 

*Corresponding author: msoerya@staff.uns.ac.id

 

 

Received: 14 September 2023; Accepted: 18 November 2023; Published:  28 February 2024

 

 

Abstract

Environmental contamination due to the disposal of poultry feather waste requires careful attention and management. As poultry feathers are a rich source of keratin protein, this study aims to determine the most efficient reducing agents for extracting β-keratin from chicken and duck feather waste as well as their optimal concentrations. For this purpose, sodium metabisulfite (Na2S2O5) and sodium dodecyl sulfate (NaC12H25SO4) at different concentrations, namely 0.2 M, 0.5 M, and       1 M, were utilized as the reducing agents. The results showed that the optimal concentration of reducing agents for extracting β-keratin from both chicken and duck feather waste was 0.2 M. Sodium metabisulfite (SMB) was proven to be the most effective reducing agent for chicken feather waste, with a yield of 76.9%. For duck feather waste, on the other hand, the use of sodium dodecyl sulfate (SDS) resulted in β-keratin extraction of 39.8%.

 

Keywords: β-keratin, feather waste, chicken feathers, duck feathers, sodium dodecyl sulfate

 

Abstrak

Pencemaran alam sekitar yang disebabkan oleh sisa bulu unggas memerlukan perhatian dan ditangani secara serius. Bulu unggas dapat dimanfaatkan sebagai salah satu sumber protein keratin. Kajian ini bertujuan untuk menentukan agen penurunan yang paling berkesan dan kepekatan optimum untuk pengekstrakan β-keratin dari sisa bulu unggas (bulu ayam dan bulu itik). Natrium metabisulfit (Na2S2O5) (SMB) dan natrium dodesil sulfat (NaC12H25SO4) (SDS) dengan pelbagai kepekatan 0.2 M, 0.5 M, dan 1 M telah digunakan sebagai agen penurunan. Kepekatan yang berkesan untuk pengekstrakan β-keratin dari sisa bulu ayan dan itik adalah 0.2 M. Agen penurunan yang berkesan untuk mengekstrak β-keratin daripada sisa bulu ayam adalah SMB menghasilkan sebanyak 76.9%.  Manakala penggunaan SDS dalam sisa bulu itik menghasilkan 39.8% β-keratin.

 

Kata kunci: β-keratin, sisa bulu, bulu ayam, bulu itik, natrium dodesil sulfat

 


References

1.    Mulia, D. S., Yuliningsih, R. T., Maryanto, H. and Purbomartono, C. (2016). Pemanfaatan limbah bulu ayam menjadi bahan pakan ikan dengan fermentasi Bacillus subtilis. Jurnal Manusia dan Lingkungan, 23(1): 49-57.

2.    Jana, A., Kakkar, N., Halder, S. K., Das, A. J., Bhaskar, T., Ray, A. and Ghosh, D. (2022). Efficient valorization of feather waste by Bacillus cereus IIPK35 for concomitant production of antioxidant keratin hydrolysate and milk-clotting metallo-serine keratinase. Journal of Environmental Management, 324: 1-11.

3.    Qiu, J., Wilkens, C., Barrett, K. and Meyer, A. S. (2020). Microbial enzymes catalyzing keratin degradation: Classification, structure, function. Biotechnology Advances, 44: 1-22.

4.    Pourjavaheri, F., Ostovar Pour, S., Jones, O. A. H., Smooker, P. M., Brkljača, R., Sherkat, F., Blanch, E. W., Gupta, A. and Shanks, R. A. (2019). Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine. Process Biochemistry, 82: 205-214.

5.    De Silva, R., Wang, X., and Byrne, N. (2016). Development of a novel cellulose/duck feather composite fibre regenerated in ionic liquid. Carbohydrate Polymers, 153: 115-123.

6.    Peng, Z., Mao, X., Zhang, J., Du, G. and Chen, J. (2019). Effective biodegradation of chicken feather waste by co-cultivation of keratinase producing strains. Microbial Cell Factories, 18(1): 1-11.

7.    Gupta, A., Kamarudin, N. B., Chua, G. K., Yunus, R. M., Yeo, C., Kee, G., Bin, R. and Yunus, M. (2012). Extraction of keratin protein from chicken feather. Journal Chemistry and Chemical Engineering, 6: 732-737.

8.    Khumalo, M., Sithole, B. and Tesfaye, T. (2020). Valorisation of waste chicken feathers: Optimisation of keratin extraction from waste chicken feathers by sodium bisulphite, sodium dodecyl sulphate and urea. Journal of Environmental Management, 262: 1-7.

9.    Sinkiewicz, I., Śliwińska, A., Staroszczyk, H. and Kołodziejska, I. (2017). Alternative methods of preparation of soluble keratin from chicken feathers. Waste and Biomass Valorization, 8(4): 1043-1048.

10. Ramya, K. R., Thangam, R. and Madhan, B. (2020). Comparative analysis of the chemical treatments used in keratin extraction from red sheep’s hair and the cell viability evaluations of this keratin for tissue engineering applications. Process Biochemistry, 90: 223-232.

11. Kamarudin, N. B., Sharma, S., Gupta, A., Kee, C. G., Chik, S. M. S. B. T. and Gupta, R. (2017). Statistical investigation of extraction parameters of keratin from chicken feather using Design-Expert. 3 Biotech, 7(2): 1-9.

12. Welu, K. T., Beyan, S. M., Balakrishnan, S., and Admassu, H. (2020). Chicken feathers based Keratin extraction process data analysis using response surface-box-Behnken design method and characterization of keratin product. Current Applied Science and Technology, 20(2): 163-177.

13. Roberts, D. W., Basketter, D., Kimber, I., White, J., McFadden, J. and White, I. R. (2012). Sodium metabisulfite as a contact allergen - An example of a rare chemical mechanism for protein modification. Contact Dermatitis, 66(3): 123-127.

14. Sarip. M, T. T. Nugroho, H. Y. T. (2014). Isolasi, uji aktivitas, dan aktivitas spesifik enzim selulase Penicillium sp. LBKURCC27 semimurni melalui pengendapan (NH4)2SO4. Jurnal Online Mahasiswa Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Riau, 1(1): 1-6.

15. Na Ayutthaya, I. S., Tanpichai, S. and Wootthikanokkhan, J. (2015). Keratin extracted from chicken feather waste: extraction, preparation, and structural characterization of the keratin and keratin/biopolymer films and electrospuns. Journal of Polymers and the Environment, 23(4): 506-516.

16. Khumalo, M., Tesfaye, T., Sithole, B.  and Ramjugernath, D. (2019). Possible beneficiation of waste chicken feathers via conversion into international journal of chemical sciences possible beneficiation of waste chicken feathers via conversion into biomedical applications. International Journal of Chemical Sciences, 17(1): 1-20.

17. Acharya, V. V. and Chaudhuri, P. (2021). Modalities of protein denaturation and nature of denaturants. International Journal of Pharmaceutical Sciences Review and Research, 69(2): 19-24.

18. Valkov, A., Zinigrad, M., Sobolev, A. and Nisnevitch, M. (2020). Keratin biomembranes as a model for studying onychomycosis. International Journal of Molecular Sciences, 21: 1-12.

19. Zhao, J., Cui, J. K., Chen, R. X., Tang, Z. Z., Tan, Z. L., Jiang, L. Y. and Liu, F. (2021). Real-time in-situ quantification of protein secondary structures in aqueous solution based on ATR-FTIR subtraction spectrum. Biochemical Engineering Journal, 176: 1-12.

20. Silaen, D. S., Herawan, T., Masyithah, Z., and Ginting, H. A. (2017). Optimasi sintesis biosurfaktan lauril amida dari asam laurat dan dietanolamina menggunakan pelarut hexane dan enzim lipase terimobilisasi optimation. Jurnal Teknik Kimia USU, 6(2): 19-23.

21. Usoltsev, D., Sitnikova, V., Kajava, A., and Uspenskaya, M. (2019). Systematic FTIR spectroscopy study of the secondary structure changes in human serum albumin under various denaturation conditions. Biomolecules, 9(8): 1-17.

22. Sadat, A. and Joye, I. J. (2020). Peak fitting applied to fourier transform infrared and raman spectroscopic analysis of proteins. Applied Sciences (Switzerland), 10(17): 2-16.

23. Shavandi, A., Bekhit, A. E. D. A., Carne, A. and Bekhit, A. (2017). Evaluation of keratin extraction from wool by chemical methods for bio-polymer application. Journal of Bioactive and Compatible Polymers, 32(2): 163-177.

24. Alashwal, B. Y., Saad Bala, M., Gupta, A., Sharma, S. and Mishra, P. (2020). Improved properties of keratin-based bioplastic film blended with microcrystalline cellulose: A comparative analysis. Journal of King Saud University - Science, 32(1): 853-857.

25. Wang, B., Yang, W., McKittrick, J. and Meyers, M. A. (2016). Keratin: Structure, mechanical properties, occurrence in biological organisms, and efforts at bioinspiration. Progress in Materials Science, 76: 229-318.

26. Alphine, J., Lucy, K. M., Maya, S., Sumena, K. B., Indu, V. R. and Vasudevan, V. N. (2019). Comparative studies on the chemical properties of feather barbs in broiler and kuttanad ducks. Journal of Veterinary and Animal Sciences, 50(2): 149-153.