Malaysian Journal of Analytical
Sciences, Vol 28
No 1 (2024): 116 - 126
EXTRACTION OF β-KERATIN FROM POULTRY FEATHER WASTE USING SODIUM
METABISULFITE AND SODIUM DODECYL SULFATE
(Pengekstrakan
β-Keratin dari Sisa Bulu Ayam Menggunakan Natrium Metabisulfit dan Natrium
Dodesil Sulfat)
Soerya Dewi Marliyana*,
Theresia Inna Sekar Tungga Dewi, and Triana Kusumaningsih
Department of Chemistry, Faculty of Mathematics and Natural
Sciences, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta 57126
*Corresponding author: msoerya@staff.uns.ac.id
Received: 14 September 2023;
Accepted: 18 November 2023; Published: 28
February 2024
Abstract
Environmental contamination due to the disposal of poultry
feather waste requires careful attention and management. As poultry feathers
are a rich source of keratin protein, this study aims to determine the most
efficient reducing agents for extracting β-keratin from chicken and duck
feather waste as well as their optimal concentrations. For this purpose, sodium
metabisulfite (Na2S2O5) and sodium dodecyl
sulfate (NaC12H25SO4) at different
concentrations, namely 0.2 M, 0.5 M, and
1 M, were utilized as the reducing agents. The results showed that the
optimal concentration of reducing agents for extracting β-keratin from
both chicken and duck feather waste was 0.2 M. Sodium metabisulfite (SMB) was
proven to be the most effective reducing agent for chicken feather waste, with
a yield of 76.9%. For duck feather waste, on the other hand, the use of sodium
dodecyl sulfate (SDS) resulted in β-keratin extraction of 39.8%.
Keywords: β-keratin, feather waste,
chicken feathers, duck feathers, sodium dodecyl sulfate
Abstrak
Pencemaran alam sekitar yang disebabkan oleh sisa bulu unggas
memerlukan perhatian dan ditangani secara serius. Bulu unggas dapat
dimanfaatkan sebagai salah satu sumber protein keratin. Kajian ini bertujuan
untuk menentukan agen penurunan yang paling berkesan dan kepekatan optimum untuk
pengekstrakan β-keratin dari sisa bulu unggas (bulu ayam dan bulu itik).
Natrium metabisulfit (Na2S2O5) (SMB) dan
natrium dodesil sulfat (NaC12H25SO4) (SDS)
dengan pelbagai kepekatan 0.2 M, 0.5 M, dan 1 M telah digunakan sebagai agen
penurunan. Kepekatan yang berkesan untuk pengekstrakan β-keratin dari sisa
bulu ayan dan itik adalah 0.2 M. Agen penurunan yang berkesan untuk mengekstrak
β-keratin daripada sisa bulu ayam adalah SMB menghasilkan sebanyak
76.9%. Manakala penggunaan SDS dalam
sisa bulu itik menghasilkan 39.8% β-keratin.
Kata
kunci: β-keratin, sisa bulu, bulu
ayam, bulu itik, natrium dodesil sulfat
References
1. Mulia, D. S.,
Yuliningsih, R. T., Maryanto, H. and Purbomartono, C. (2016). Pemanfaatan limbah bulu ayam menjadi
bahan pakan ikan dengan fermentasi Bacillus subtilis. Jurnal Manusia dan Lingkungan, 23(1): 49-57.
2. Jana, A., Kakkar,
N., Halder, S. K., Das, A. J., Bhaskar, T., Ray, A. and Ghosh, D. (2022).
Efficient valorization of feather waste by Bacillus cereus IIPK35 for
concomitant production of antioxidant keratin hydrolysate and milk-clotting
metallo-serine keratinase. Journal of Environmental Management, 324:
1-11.
3. Qiu, J., Wilkens,
C., Barrett, K. and Meyer, A. S. (2020). Microbial enzymes catalyzing keratin
degradation: Classification, structure, function. Biotechnology Advances,
44: 1-22.
4. Pourjavaheri, F.,
Ostovar Pour, S., Jones, O. A. H., Smooker, P. M., Brkljača, R., Sherkat,
F., Blanch, E. W., Gupta, A. and Shanks, R. A. (2019). Extraction of keratin
from waste chicken feathers using sodium sulfide and L-cysteine. Process
Biochemistry, 82: 205-214.
5. De Silva, R.,
Wang, X., and Byrne, N. (2016). Development of a novel cellulose/duck feather
composite fibre regenerated in ionic liquid. Carbohydrate Polymers, 153:
115-123.
6. Peng, Z., Mao, X.,
Zhang, J., Du, G. and Chen, J. (2019). Effective biodegradation of chicken
feather waste by co-cultivation of keratinase producing strains. Microbial
Cell Factories, 18(1): 1-11.
7. Gupta, A.,
Kamarudin, N. B., Chua, G. K., Yunus, R. M., Yeo, C., Kee, G., Bin, R. and
Yunus, M. (2012). Extraction of keratin protein from chicken feather. Journal
Chemistry and Chemical Engineering, 6: 732-737.
8. Khumalo, M.,
Sithole, B. and Tesfaye, T. (2020). Valorisation of waste chicken feathers:
Optimisation of keratin extraction from waste chicken feathers by sodium
bisulphite, sodium dodecyl sulphate and urea. Journal of Environmental
Management, 262: 1-7.
9. Sinkiewicz, I.,
Śliwińska, A., Staroszczyk, H. and Kołodziejska, I. (2017).
Alternative methods of preparation of soluble keratin from chicken feathers. Waste
and Biomass Valorization, 8(4): 1043-1048.
10. Ramya, K. R.,
Thangam, R. and Madhan, B. (2020). Comparative analysis of the chemical
treatments used in keratin extraction from red sheep’s hair and the cell
viability evaluations of this keratin for tissue engineering applications. Process
Biochemistry, 90: 223-232.
11. Kamarudin, N. B.,
Sharma, S., Gupta, A., Kee, C. G., Chik, S. M. S. B. T. and Gupta, R. (2017).
Statistical investigation of extraction parameters of keratin from chicken
feather using Design-Expert. 3 Biotech, 7(2): 1-9.
12. Welu, K. T., Beyan,
S. M., Balakrishnan, S., and Admassu, H. (2020). Chicken feathers based Keratin
extraction process data analysis using response surface-box-Behnken design
method and characterization of keratin product. Current Applied Science and
Technology, 20(2): 163-177.
13. Roberts, D. W.,
Basketter, D., Kimber, I., White, J., McFadden, J. and White, I. R. (2012).
Sodium metabisulfite as a contact allergen - An example of a rare chemical
mechanism for protein modification. Contact Dermatitis, 66(3): 123-127.
14. Sarip. M, T. T.
Nugroho, H. Y. T. (2014). Isolasi, uji aktivitas, dan aktivitas spesifik enzim
selulase Penicillium sp. LBKURCC27 semimurni melalui pengendapan (NH4)2SO4.
Jurnal Online Mahasiswa Fakultas Matematika dan Ilmu Pengetahuan Alam
Universitas Riau, 1(1): 1-6.
15. Na Ayutthaya, I.
S., Tanpichai, S. and Wootthikanokkhan, J. (2015). Keratin extracted from
chicken feather waste: extraction, preparation, and structural characterization
of the keratin and keratin/biopolymer films and electrospuns. Journal of
Polymers and the Environment, 23(4): 506-516.
16. Khumalo, M.,
Tesfaye, T., Sithole, B. and
Ramjugernath, D. (2019). Possible beneficiation of waste chicken feathers via
conversion into international journal of chemical sciences possible
beneficiation of waste chicken feathers via conversion into biomedical
applications. International Journal of Chemical Sciences, 17(1): 1-20.
17. Acharya, V. V. and
Chaudhuri, P. (2021). Modalities of protein denaturation and nature of
denaturants. International Journal of Pharmaceutical Sciences Review and
Research, 69(2): 19-24.
18. Valkov, A.,
Zinigrad, M., Sobolev, A. and Nisnevitch, M. (2020). Keratin biomembranes as a
model for studying onychomycosis. International Journal of Molecular
Sciences, 21: 1-12.
19. Zhao, J., Cui, J.
K., Chen, R. X., Tang, Z. Z., Tan, Z. L., Jiang, L. Y. and Liu, F. (2021).
Real-time in-situ quantification of protein secondary structures in aqueous
solution based on ATR-FTIR subtraction spectrum. Biochemical Engineering
Journal, 176: 1-12.
20. Silaen, D. S.,
Herawan, T., Masyithah, Z., and Ginting, H. A. (2017). Optimasi sintesis biosurfaktan lauril
amida dari asam laurat dan dietanolamina menggunakan pelarut hexane dan enzim
lipase terimobilisasi optimation. Jurnal
Teknik Kimia USU, 6(2):
19-23.
21. Usoltsev, D.,
Sitnikova, V., Kajava, A., and Uspenskaya, M. (2019). Systematic FTIR
spectroscopy study of the secondary structure changes in human serum albumin
under various denaturation conditions. Biomolecules, 9(8): 1-17.
22. Sadat, A. and Joye,
I. J. (2020). Peak fitting applied to fourier transform infrared and raman
spectroscopic analysis of proteins. Applied Sciences (Switzerland),
10(17): 2-16.
23. Shavandi, A.,
Bekhit, A. E. D. A., Carne, A. and Bekhit, A. (2017). Evaluation of keratin
extraction from wool by chemical methods for bio-polymer application. Journal
of Bioactive and Compatible Polymers, 32(2): 163-177.
24. Alashwal, B. Y.,
Saad Bala, M., Gupta, A., Sharma, S. and Mishra, P. (2020). Improved properties
of keratin-based bioplastic film blended with microcrystalline cellulose: A
comparative analysis. Journal of King Saud University - Science, 32(1):
853-857.
25. Wang, B., Yang, W.,
McKittrick, J. and Meyers, M. A. (2016). Keratin: Structure, mechanical
properties, occurrence in biological organisms, and efforts at bioinspiration. Progress
in Materials Science, 76: 229-318.
26. Alphine, J., Lucy,
K. M., Maya, S., Sumena, K. B., Indu, V. R. and Vasudevan, V. N. (2019).
Comparative studies on the chemical properties of feather barbs in broiler and
kuttanad ducks. Journal of Veterinary and Animal Sciences, 50(2):
149-153.