Malaysian Journal of Analytical Sciences Vol 25 No 2 (2021): 341 - 351

 

 

 

 

Mode of Action of 5-Acetyl-4-Methylthiazole Derivatives as Antimicrobial Agents

 

(Mod Tindakan Sebatian Terbitan 5-Asetil-4-Metiltiazol Sebagai Agen Antimikrob)

 

Iswatun Hasanah Abdullah Ripain, Nurziana Ngah*, Deny Susanti Darnis

 

Department of Chemistry, Kulliyyah of Science,

International Islamic University Malaysia, Kuantan Campus, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia

 

^*Corresponding author:  nurziana@iium.edu.my

 

 

Received: 5 February 2021; Accepted: 29 March 2021; Published:  25 April 2021

 

 

Abstract

Thiazole derivatives have been widely known to possess antimicrobial behaviour. The purpose of this study is to investigate the mode of antimicrobial actions of synthesised thiazole compounds on Gram-positive bacteria (Staphylococcus aureus), Gram-negative bacteria (Salmonella typhimurium), and fungus (Candida albicans). Three synthesised thiazole compounds namely 5-acetyl-4-methyl-2-(3,4-dichloroaniline)-1,3-thiazole (T1), 5-acetyl-4-methyl-2-(4-aminophenol)-1,3-thiazole (T2), and 5-acetyl-4-methyl-2-(methyl-4-aminobenzoate)-1,3-thiazole (T3) were evaluated in the mode of action assays such as salt tolerance, time-killing, crystal violet and leakage 260/280 nm absorbing materials. The result showed that the T3 compound exhibited the best performance for all tested assays at a concentration equal to 4 × MIC compared to T1 and T2.

 

Keywords:  thiazole, mode of action, antimicrobial

 

Abstrak

Terbitan tiazol telah dikenali umum sebagai sebatian yang mempunyai sifat antimikrob. Justeru, kajian ini dijalankan untuk mengkaji mod tindakan sebatian tiazol yang disintesis sebagai agen antimikrob terhadap bakteria Gram-positif (Staphylococcus aureus), Gram-negatif (Salmonella typhimurium) and fungus (Candida albicans). Tiga sebatian tiazol yang telah disintesis iaitu 5-asetil-4-metil-2-(3,4-dikloroanilina)-1,3-tiazol (T1), 5-asetil-4-metil-2-(4-aminofenol)-1,3-tiazol (T2) and 5-asetil-4-metil-2-(metil-4-aminobenzoat)-1,3-tiazol (T3) telah dikaji dan dinilai secara toleransi garam, masa pembunuhan, violet kristal dan kebocoran bahan serapan 260/280 nm. Keputusan kajian mendapati sebatian T3 menunjukkan kebolehan sebagai agen antimikrob yang terbaik pada kepekatan 4 × MIC berbanding T1 dan T2.

 

Kata kunci:  tiazol, mod tindakan, antimikrob

 

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