Malaysian
Journal of Analytical Sciences Vol 23 No 6 (2019): 991 - 1000
DOI:
10.17576/mjas-2019-2306-07
MODIFICATION OF GOLD SCREEN PRINTED
ELECTRODE FOR THE DETECTION OF TOXIC DOMOIC ACID
(Pengubahsuaian
Elektrod Cetakan Skrin Emas bagi Pengesanan Asid Domoik Toksik)
Hafiza Mohamed
Zuki*, Norhidayah Mohd Nasri, Fatin Nabilah Muhamad, Noor Sheryna Jusoh, Azrilawani
Ahmad, Marinah Mohd Ariffin
Faculty
of Science and Marine Environment,
Universiti
Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
*Corresponding author: hafiza@umt.edu.my
Received: 30 October 2018;
Accepted: 26 September 2019
Abstract
Entrapped
ninhydrin in polyvinylchloride (PVC) film is directly immobilized on the
surface of gold screen printed electrodes (Au-SPE). Ninhydrin acts as a
potential reagent for the detection of toxic Domoic Acid (DA) on the modified
Au-SPE sensing system. The modified Au-SPE was characterized using cyclic
voltammetry (CV), where the electrochemical behavior of ninhydrin-DA on
modified Au-SPE surface was investigated in the potential range of -0.6 V to
+0.85 V at a 50 mVs-1 scan rate, in the presence of 10 mM potassium
ferricyanide in 0.1 M potassium chloride (KCl). Good responses were observed
for ninhydrin-DA redox reactions with a linear relationship forming between
peak currents and concentrations. The obtained limit of detection (LOD) for the
sensing system was 7.64 x 10-5 M. The developed sensing system of
modified Au-SPE exhibited excellent reproducibility with RSD obtained between
0.8% to 1.2%.
Keywords: domoic acid, ninhydrin, modified gold screen
printed electrode, cyclic voltammetry
Abstrak
Ninhidrin yang diperangkap dalam lapisan filem
polivinilklorida (PVC) dipegunkan secara langsung pada permukaan elektrod
cetakan skrin emas (Au-SPE). Ninhidrin bertindak sebagai reagen yang berpotensi
untuk mengesan asid domoik (DA) toksik diatas sistem pengesan Au-SPE yang
diubahsuai. Au-SPE yang diubahsuai dicirikan dengan menggunakan voltametri
kitaran (CV) di mana sifat elektrokimia ninhidrin-DA pada permukaan Au-SPE yang
diubahsuai telah dikaji dalam julat keupayaan antara -0.6 hingga +0.85 V pada
kadar imbasan 50 mVs-1 dalam kehadiran 10 mM kalium ferisianida
dalam 0.1 M kalium klorida (KCl). Rangsangan yang baik telah dicerap bagi
tindak balas redoks ninhidrin-DA dengan hubungan linear diperoleh antara arus
puncak dan kepekatan. Had pengesanan (LODs) yang diperolehi bagi system
pengesan adalah 7.64 x 10-5 M. Sistem pengesan Au-SPE diubahsuai
yang dibangunkan, mempamerkan kebolehulangan yang sangat baik dengan julat
nilai RSD diperolehi antara 0.8% hingga 1.2%.
Kata kunci: asid domoik,
ninhidrin, elektrod cetakan skrin emas, voltametri kitaran
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