Malaysian Journal of Analytical Sciences Vol 22 No 4
(2018): 676 - 683
DOI:
10.17576/mjas-2018-2204-14
BIOSYNTHESIS OF SILVER
NANOPARTICLES USING Citrus grandis PEEL EXTRACT
(Biosintesis Nanopartikel
Perak dengan Menggunakan Ekstrak Kulit Citrus
grandis)
Nur Syazana Jalani1,
Wong Michell1, Wong Ee Lin1, Sharifah Zati Hanani1*,
Uda Hashim2, Rozaini Abdullah1
1Department of Chemical Engineering, Faculty of Engineering Technology,
Universiti Malaysia Perlis,
Kampus UniCITI Alam, 02100 Sungai Chuchuh, Padang Besar, Perlis, Malaysia
2Institute Nano Electronic Engineering,
Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia
*Corresponding author: sharifahzati@unimap.edu.my
Received: 16
April 2017; Accepted: 7 March 2018
Abstract
In this study, Citrus
grandis or pomelo peel extract was used to synthesize silver nanoparticles
(AgNPs). The effect of reaction time, amount of plant extract and concentration
of silver nitrate which could influence the biosynthesis process were also
studied. The synthesized AgNPs were then characterized by UV-Visible
spectroscopy, Fourier transform infra-red spectroscopy (FTIR) and field
emission scanning microscopy (FESEM) equipped with energy dispersive X-ray
(EDX). The resulting UV-Vis spectra of synthesized AgNPs from pomelo peel
extract showed standard surface plasmon resonance band in range of 401-433 nm
which indicated the presence of AgNPs. FTIR spectra confirmed the existence of
possible functional groups which might be responsible for the reduction of
nanoparticles. Most of the synthesized AgNPs were observed in spherical shape
which ranged between 20-30 nm under the Field Emission Scanning Electron
microscope. The EDX analysis showed the strong signal at 3 keV from the silver
atoms in the synthesized nanoparticles which confirmed that the synthesis of
AgNPs was successful.
Keywords: silver nanoparticles, Citrus grandis peel extract, biosynthesis, surface plasmon
resonance
Abstrak
Dalam kajian ini, ekstrak kulit Citrus grandis
atau limau bali telah digunakan untuk menghasilkan nanopartikel perak. Pengaruh
masa tindak balas, jumlah ekstrak kulit limau bali dan kepekatan perak nitrat
ke atas biosintesis nanopartikel perak turut dikaji. Pencirian nanopartikel
perak yang terbentuk kemudiannya dijalankan menggunakan spektroskopi sinar UV,
spektroskopi infra merah transformasi Fourier (FTIR) dan mikroskopi imbasan pancaran medan (FESEM) dilengkapi
dengan sinar-X tenaga serakan (EDX). Spektra UV-Vis
nanopartikel perak dari ektrak kulit limau bali menunjukkan puncak resonansi
plasmon sekitar 401-433 nm yang membuktikan pembentukan nanopartikel perak.
Spektra FTIR membuktikan kewujudan beberapa kumpulan berfungsi yang mungkin
terlibat dalam proses penurunan nanopartikel. Kebanyakan nanopartikel perak
dilihat berbentuk sfera dan bersaiz di antara 20-30 nm melalui mikroskopi imbasan pancaran medan. Analisis EDX pula menunjukkan isyarat yang kuat
daripada atom perak dalam nanopartikel yang telah disintesis dan ini
membuktikan sintesis nanopartikel perak telah berjaya.
Kata kunci: nanopartikel perak, ekstrak
kulit Citrus grandis, biosintesis, puncak resonansi plasmon
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