Malaysian Journal of Analytical Sciences Vol 20 No 6 (2016): 1233 - 1240

DOI: http://dx.doi.org/10.17576/mjas-2016-2006-01

 

 

 

STUDIES OF INTERACTION BETWEEN TETRABUTYLAMMONIUM BROMIDE BASED DEEP EUTECTIC SOLVENT AND DNA USING FLUORESCENCE QUENCHING METHOD AND CIRCULAR DICHROISM SPECTROSCOPY

 

(Kajian Interaksi antara Pelarut Eutektik Mendalam Berasaskan Tetrabutilamonium Bromida dan DNA Menggunakan Kaedah Pelindapkejutan Pendarfluor dan Spektroskopi Edaran Dikroisme)

 

Rizana Yusof1, Haslina Ahmad1,2, Mohd Basyaruddin Abdul Rahman1,2*

 

1Department of Chemistry, Faculty of Science

2Enzyme and Microbial Technology Research Centre (EMTech)

Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia

 

*Corresponding author: basya@upm.edu.my

 

 

Received: 17 August 2015; Accepted: 17 August 2016

 

 

Abstract

The interaction of tetrabutylammonium bromide (TBABr)-based deep eutectic solvents (DESs) with calf thymus DNA (ct-DNA) was investigated by fluorescence and circular dichroism (CD) spectroscopy. The influences of hydrogen bond donors (HBDs), with respect to chain lengths and the presence of OH group were determined through ethidium bromide (EB) fluorescence quenching. Based on the quenching process, it was concluded that hydrophobic interactions were responsible for the DES ability to quench EB from EB-bound DNA. The strong electrostatic attraction between TBA+ cation on DES and negative charge phosphate DNA was proved from fluorescence and CD spectroscopy. DNA was able to maintain its double helical structure at 25 °C with a concentration of 25% DES.

 

Keywords:    binding, deep eutectic solvent, tetrabutylammonium bromide, fluorescence quenching, circular dichroism spectroscopy

 

Abstrak

Ikatan antara pelarut eutektik berasaskan tetrabutilammonium bromida (TBABr) dengan ‘calf thymus’ DNA (ct-DNA) telah diuji dengan spektroskopi pendarfluor dan edaran dikroisme (CD). Pengaruh penderma ikatan hidrogen (HBDs) berdasarkan panjang rantai dan kehadiran kumpulan OH telah ditentukan melalui pelindapkejutan etidium bromida (EB). Berdasarkan proses pelindapkejutan, dapat dirumuskan bahawa ikatan hidrofobik berperanan dalam menentukan keupayaan DES untuk menyingkirkan EB daripada EB-terikat DNA. Tarikan elektrostatik yang kuat antara kation TBA+ pada DES dengan cas negatif fosfat DNA telah dibuktikan daripada kedua-dua spektroskopi pendarfluor dan CD. DNA berupaya mengekalkan struktur heliks berganda pada suhu 25 °C dan kepekatan DES pada 25%.

 

Katakunci:     ikatan, pelarut eutektik mendalam, tetrabutilammonium bromida, pelindapkejutan pendarfluor, spektroskopi edaran dikroisme

 

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