Malaysian Journal of Analytical Sciences Vol 20 No 3 (2016): 525 - 530

DOI: http://dx.doi.org/10.17576/mjas-2016-2003-09

 

 

 

XYLOOLIGOSACCHARIDES PRODUCTION FROM OIL PALM FROND BY Trichoderma longibrachiatum XYLANASE

 

(Penghasilan Xilooligosakarida daripada Pelepah Kelapa Sawit Menggunakan Xilanase Trichoderma Longibrachiatum)

 

Sabiha Hanim Saleh1*, Siti Normah Mohd Damanhuri Shah1, Khalilah Abdul Khalil2, Aishah Bujang3

 

1School of Chemistry and Environment

2School of Biology

3School of Food Science and Technology

Faculty of Applied Sciences,

Universiti Teknologi MARA, 40450 Shah Alam, Selangor Malaysia

 

*Corresponding author: sabihahanim@salam.uitm.edu.my

 

 

Received: 24 February 2015; Accepted: 27 October 2015

 

 

Abstract

Oil palm fronds containing rich hemicellulose are low cost resources that could be potentially converted into valuable products such as xylooligosaccharides. The main objective of this study was to investigate the production of xylooligosaccharides from OPF hemicelluloses using Trichoderma longibrachiatum xylanase. The OPF hemicellulose extracted by alkaline extraction was hydrolysed by xylanase at pH 4.6, temperature 40 °C, hemicellulose substrate concentration 2 % (w/v) and enzyme concentration 2 U/ml for different period of time from 0 to 48 hours to produce xylooligosaccharides. The hydrolysate obtained from enzymatic hydrolysis was further purified through ultrafiltration using 10 kDa molecular weight cut off membranes. The highest total of xylobiose and xylotriose was found to be 21.91 mg/mL and obtained at 8 hours of hydrolysis time. After ultrafiltration step, xylooligosaccharides mixture were obtained in the permeate and retentate. The highest xylobiose (56.64g/100g) and xylotriose (45.80g/100g) were found in retentate and permeate, respectively.

 

Keywords:  xylooligosaccharides, oil palm frond, ultrafiltration, enzymatic hydrolysis, xylanase

 

Abstrak

Pelepah kelapa sawit  yang kaya dengan hemisellulosa adalah sumber kos rendah yang berpotensi untuk ditukarkan kepada produk bernilai seperti xilooligosakarida. Objektif utama kajian ini adalah untuk menyiasat penghasilan xilooligosakarida daripada hemisellulosa pelepah kelapa sawit menggunakan enzim  xilanase Trichoderma longibrachiatum. Hemisellulosa yang diekstrak daripada pelepah kelapa sawit menggunakan alkali, dihidrolisis menggunakan enzim xilanase pada pH 4.6, suhu 40 °C, kepekatan hemiselulose 2 % (w/v) dan kepekatan enzim 2U/ml umtuk tempoh masa berbeza dari 0 hingga 48 jam untuk menghasilkan xilooligosakarida. Hidrolisat yang diperoleh daripada hidrolisis enzimatik kemudiannya ditulenkan melalui ultrafiltrasi menggunakan membran yang berat molekulnya 10 kDa. Jumlah tertinggi xilobios dan xilotrios adalah 21.91 mg/mL yang diperoleh pada masa hidrolisis 8 jam. Setelah ultrafiltrasi, campuran xilooligosakarida diperoleh dalam ‘permeate’ dan ‘retentate’. Kandungan tertinggi xilobios (56.64g/100g) dan xilotrios (45.80g/100g) ditemui di dalam ‘retentate’ dan ‘permeate’.

 

Kata kunci: xilooligosakarida, pelepah kelapa sawit, ultrafiltrasi, hidrolisis enzimatik, xilanase

 

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