Malaysian
Journal of Analytical Sciences Vol 20 No 3 (2016): 525 - 530
DOI:
http://dx.doi.org/10.17576/mjas-2016-2003-09
XYLOOLIGOSACCHARIDES
PRODUCTION FROM OIL PALM FROND BY Trichoderma longibrachiatum XYLANASE
(Penghasilan
Xilooligosakarida daripada Pelepah Kelapa Sawit Menggunakan Xilanase Trichoderma
Longibrachiatum)
Sabiha
Hanim Saleh1*, Siti Normah Mohd Damanhuri Shah1, Khalilah
Abdul Khalil2, Aishah Bujang3
1School of Chemistry and Environment
2School of Biology
3School of Food Science and Technology
Faculty of Applied Sciences,
Universiti Teknologi MARA, 40450 Shah Alam, Selangor Malaysia
*Corresponding author: sabihahanim@salam.uitm.edu.my
Received: 24
February 2015; Accepted: 27 October 2015
Abstract
Oil palm fronds containing rich hemicellulose are low cost
resources that could be potentially converted into valuable products such as xylooligosaccharides. The main objective of this study was to investigate the production of
xylooligosaccharides from OPF hemicelluloses using Trichoderma longibrachiatum xylanase. The OPF hemicellulose
extracted by alkaline extraction was hydrolysed by xylanase at pH 4.6,
temperature 40 °C, hemicellulose substrate concentration 2 % (w/v) and enzyme
concentration 2 U/ml for different period of time from 0
to 48 hours to produce xylooligosaccharides. The hydrolysate obtained from
enzymatic hydrolysis was further purified through ultrafiltration using 10 kDa
molecular weight cut off membranes. The highest
total of xylobiose and xylotriose was found to be 21.91 mg/mL and obtained at 8
hours of hydrolysis time. After ultrafiltration
step, xylooligosaccharides mixture were obtained in the permeate and retentate.
The highest xylobiose (56.64g/100g) and
xylotriose (45.80g/100g) were found in
retentate and permeate, respectively.
Keywords: xylooligosaccharides,
oil palm frond, ultrafiltration, enzymatic hydrolysis, xylanase
Abstrak
Pelepah kelapa sawit yang kaya dengan hemisellulosa adalah sumber
kos rendah yang berpotensi untuk ditukarkan kepada produk bernilai seperti
xilooligosakarida. Objektif utama kajian ini adalah untuk menyiasat penghasilan
xilooligosakarida daripada hemisellulosa pelepah kelapa sawit menggunakan enzim
xilanase Trichoderma longibrachiatum. Hemisellulosa yang diekstrak daripada pelepah kelapa
sawit menggunakan alkali, dihidrolisis menggunakan enzim xilanase pada pH 4.6, suhu 40 °C,
kepekatan hemiselulose 2 % (w/v) dan kepekatan enzim 2U/ml umtuk tempoh masa berbeza dari 0 hingga 48 jam untuk
menghasilkan xilooligosakarida. Hidrolisat yang diperoleh daripada hidrolisis
enzimatik kemudiannya ditulenkan melalui ultrafiltrasi menggunakan membran yang
berat molekulnya 10 kDa. Jumlah tertinggi xilobios dan xilotrios adalah 21.91 mg/mL yang
diperoleh pada masa hidrolisis 8 jam. Setelah ultrafiltrasi, campuran xilooligosakarida
diperoleh dalam ‘permeate’ dan ‘retentate’. Kandungan tertinggi xilobios (56.64g/100g) dan xilotrios (45.80g/100g)
ditemui di dalam ‘retentate’ dan ‘permeate’.
Kata kunci: xilooligosakarida, pelepah kelapa sawit, ultrafiltrasi,
hidrolisis enzimatik, xilanase
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