Malaysian Journal of Analytical Sciences Vol 20 No 3
(2016): 500 - 509
DOI:
http://dx.doi.org/10.17576/mjas-2016-2003-06
NITROGEN USE
EFFICIENCY IN MR219-4 AND MR219-9 RICE MUTANT LINES UNDER DIFFERENT WATER POTENTIALS AND NITROGEN LEVELS USING 15N ISOTOPIC TRACER
TECHNIQUE
(Kecekapan
Penggunaan Nitrogen dalam Padi Titisan Mutan MR219-4 dan MR219-9 di bawah
Potensi Air dan Aras Nitrogen yang Berbeza Menggunakan Teknik Isotop Penyurih 15N)
Ahmad Nazrul Abd Wahid1, 2*, Sahibin
Abdul Rahim1, Khairuddin Abdul Rahim2, Abdul Rahim Harun2
1Environmental Science Programme,
School of
Environmental and Natural Resource Science, Faculty of Science and Technology,
Universiti
Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
2Agrotechnology and Bioscience Division,
Malaysian Nuclear
Agency, 43000 Kajang, Bangi, Selangor, Malaysia
*Corresponding author: a_nazrul@nuclearmalaysia.gov.my
Received: 3
August 2015; Accepted: 29 March 2016
Abstract
This study was conducted to evaluate the nitrogen use efficiency of rice mutant lines of MR219, viz. MR219-4 and MR219-9, developed
under rice radiation mutagenesis programme for adaptability to aerobic
conditions, grown under conditions of
varying water potentials and
nitrogen levels in a shade house. Direct 15N isotopic tracer method
was used in this study, whereby the 15N labeled
urea fertilizer was utilized as a tracer for nitrogen nutrient uptake
by the test crops. 15N
isotope presence in
the samples was determined by using emission
spectrometer analysis and percentage of
total nitrogen was determined by using Kjeldahl method. 15N atom access values of the samples were to determine the efficiency of N
use by the mutant varieties. In this work, the data of yield components,
viz. grain yield, plant biomass, plant height and 1000 grain
weight, and nitrogen components viz,
total N, %
nitrogen derived from labeled fertilizer (% Ndff), % nitrogen derived
from soil (% Ndfs), nitrogen uptake, fertilizer nitrogen use and
nitrogen use efficiency were obtained. Results from this study showed a
significant difference to the
nitrogen component and yield when there is a change in the rate of
fertilizer and water
potential. However, varietal
factor does not show any significant differences.
Keywords: mutant lines of rice, aerobic condition, water potentials, 15N isotopic tracer,
nitrogen use efficiency
Abstrak
Kajian
ini telah dijalankan untuk menilai keberkesanan penggunaan
nitrogen padi garisan mutan dari MR219, iaitu MR219-4 dan MR219-9, yang
dibangunkan di bawah program mutagenesis untuk penyesuaian ke
keadaan aerob dan ditanam dalam keadaan potensi air dan aras nitrogen yang
berbeza di dalam rumah teduhan.
Kaedah isotop penyurih 15N
secara terus telah digunakan dalam
kajian ini, di mana baja urea berlabel isotop
15N digunakan sebagai penyurih untuk pengambilan
nutrien nitrogen oleh tanaman ujian. Kehadiran isotop 15N dalam sampel ditentukan dengan analisis spektrometer pelepasan
dan peratusan jumlah nitrogen di dalam sampel ditentukan dengan menggunakan kaedah Kjeldahl. Nilai
akses Atom 15N
yang terkandung dalam sampel akan digunakan dalam penentuan keberkesanan penggunaan nitrogen dalam baja melalui
formula pengiraan tertentu. Melalui kajian ini, data daripada komponen hasil
(hasil bijirin, berat biojisim, ketinggian tumbuhan dan 1000 berat bijirin) dan
komponen nitrogen (jumlah
nitrogen, % Ndff, % Ndfs, pengambilan nitrogen, penggunaan baja nitrogen dan
kecekapan penggunaan nitrogen) telah diperolehi. Hasil daripada
kajian ini telah menunjukkan terdapat perbezaan yang ketara terhadap komponen
nitrogen dan hasil tanaman apabila terdapat perubahan faktor kadar baja dan
potensi air. Walaubagaimanapun, faktor varieti tidak menunjukkan apa – apa
perbezaan yang ketara.
Kata kunci: titisan mutan
padi, keadaan aerob, potensi air, isotop penyurih 15N, kecekapan
penggunaan nitrogen
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