Malaysian Journal of Analytical Sciences Vol 20 No 3 (2016): 500 - 509

DOI: http://dx.doi.org/10.17576/mjas-2016-2003-06

 

 

 

NITROGEN USE EFFICIENCY IN MR219-4 AND MR219-9 RICE MUTANT LINES UNDER DIFFERENT WATER POTENTIALS AND NITROGEN LEVELS USING 15N ISOTOPIC TRACER TECHNIQUE

 

(Kecekapan Penggunaan Nitrogen dalam Padi Titisan Mutan MR219-4 dan MR219-9 di bawah Potensi Air dan Aras Nitrogen yang Berbeza Menggunakan Teknik Isotop Penyurih 15N)

 

Ahmad Nazrul Abd Wahid1, 2*, Sahibin Abdul Rahim1, Khairuddin Abdul Rahim2, Abdul Rahim Harun2

 

1Environmental Science Programme,

School of Environmental and Natural Resource Science, Faculty of Science and Technology,

Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

2Agrotechnology and Bioscience Division,

Malaysian Nuclear Agency, 43000 Kajang, Bangi, Selangor, Malaysia

 

*Corresponding author: a_nazrul@nuclearmalaysia.gov.my

 

 

Received: 3 August 2015; Accepted: 29 March 2016

 

 

Abstract

This study was conducted to evaluate the nitrogen use efficiency of rice mutant lines of MR219, viz. MR219-4 and MR219-9, developed under rice radiation mutagenesis programme for adaptability to aerobic conditions, grown under conditions of varying water potentials and nitrogen levels in a shade house. Direct 15N isotopic tracer method was used in this study, whereby the 15N labeled urea fertilizer was utilized as a tracer for nitrogen nutrient uptake by the test crops. 15N isotope presence in the samples was determined by using emission spectrometer analysis and percentage of total nitrogen was determined by using Kjeldahl method. 15N atom access values of the samples were to determine the efficiency of N use by the mutant varieties. In this work, the data of yield components, viz. grain yield, plant biomass, plant height and 1000 grain weight, and nitrogen components viz, total N, % nitrogen derived from labeled fertilizer (% Ndff), % nitrogen derived from soil (% Ndfs), nitrogen uptake, fertilizer nitrogen use and nitrogen use efficiency were obtained. Results from this study showed a significant difference to the nitrogen component and yield when there is a change in the rate of fertilizer and water potential. However, varietal factor does not show any significant differences.

 

Keywords:  mutant lines of rice, aerobic condition, water potentials, 15N isotopic tracer, nitrogen use efficiency

 

Abstrak

Kajian ini telah dijalankan untuk menilai keberkesanan penggunaan nitrogen padi garisan mutan dari MR219, iaitu MR219-4 dan MR219-9, yang dibangunkan di bawah program mutagenesis untuk penyesuaian ke keadaan aerob dan ditanam dalam keadaan potensi air dan aras nitrogen yang berbeza di dalam rumah teduhan. Kaedah isotop penyurih 15N secara terus telah digunakan dalam kajian ini, di mana baja urea berlabel isotop 15N digunakan sebagai penyurih untuk pengambilan nutrien nitrogen oleh tanaman ujian. Kehadiran isotop 15N dalam sampel ditentukan dengan analisis spektrometer pelepasan dan peratusan jumlah nitrogen di dalam sampel ditentukan dengan menggunakan kaedah Kjeldahl. Nilai akses Atom 15N yang terkandung dalam sampel akan digunakan dalam penentuan keberkesanan penggunaan nitrogen dalam baja melalui formula pengiraan tertentu. Melalui kajian ini, data daripada komponen hasil (hasil bijirin, berat biojisim, ketinggian tumbuhan dan 1000 berat bijirin) dan komponen nitrogen (jumlah nitrogen, % Ndff, % Ndfs, pengambilan nitrogen, penggunaan baja nitrogen dan kecekapan penggunaan nitrogen) telah diperolehi. Hasil daripada kajian ini telah menunjukkan terdapat perbezaan yang ketara terhadap komponen nitrogen dan hasil tanaman apabila terdapat perubahan faktor kadar baja dan potensi air. Walaubagaimanapun, faktor varieti tidak menunjukkan apa – apa perbezaan yang ketara.

 

Kata kunci: titisan mutan padi, keadaan aerob, potensi air, isotop penyurih 15N, kecekapan penggunaan nitrogen

 

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