Malaysian
Journal of Analytical Sciences Vol 20 No 2 (2016): 231 - 237
PENGGUNAAN KAEDAH PENYINARAN MIKROGELOMBANG DALAM
SINTESIS TERBITAN TIOUREA
(An
Application of Microwave
Irradiation Technique in Preparation of Thiourea Derivatives)
Hafizi Ahyak, Atisya
Rohadi, Fatimatul Akma Awang Ngah, Bohari Yamin, Siti Aishah Hasbullah
School of
Chemical Sciences and Food Technology,
Faculty
of Science and Technology,
Universiti
Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
*Corresponding author: aishah80@ukm.edu.my
Received:
22 January 2016; Accepted: 18 February 2016
Abstrak
Sebatian
baru terbitan tiourea, 1-etil-1-(2-hidroksi-etil)-3-naftalen-1-il-tiourea (3a) dan
1-(fenil-amino)-3-naftalen-1-il-tiourea (3b)
telah berjaya disintesis melalui tindak balas antara 1-naftil isotiosianat (1) dengan etilena 2-etilaminoetanol (2a) dan fenilhidrazina (2b) menggunakan kaedah penyinaran
mikrogelombang dan kaedah refluks. Peratusan hasil bagi kedua-dua kaedah yang
digunakan untuk sebatian 3a dan 3b masing-masing adalah 3a (refluks: 66%, mikrogelombang: 98%) dan 3b (refluks: 72%, mikrogelombang: 97%). Hasil
sintesis dianalisa dengan menggunakan alat spektroskopi FT-Inframerah (FT-IM),
Spektroskopi Resonan Magnetik Nuklear (RMN) dan Spektrometri Jisim. Kajian
antimikrob juga dilakukan dengan menggunakan Staphylococcus aureus dan
Bacillus subtillus sebagai Gram positif, Escherichia coli dan Salmonella
typhimurium sebagai Gram negatif dan kulat yang digunakan adalah Aspergillus niger. Kawalan positif yang
digunakan adalah streptomisin dan kanamisin, manakala kawalan negatif yang
digunakan adalah DMSO. Walaubagaimanapun hasil kajian mendapati tiada
perencatan yang ketara terhadap pertumbuhan bakteria dan kulat (hanya 6-7mm
kawasan perencatan) menggunakan sebatian tiourea ini.
Kata kunci: sebatian tiourea, mikrogelombang, aktiviti antimikrob
Abstract
New
thiourea derivatives, 1-ethyl-1-(2-hydroxy-ethyl)-3-naphthalen-1-yl-iourea (3a),
1-(phenyl-amino)-3-naphthalen-1-yl-Tiourea (3b)
have been synthesized from the reaction of naphthylisothiocyanate with
2-(ethylamino)ethanol (2a) and
phenylhydrazine (2b) using microwave
irradiation method. The percentage yield for both method used for compound 3a and 3b, respectively 3a (reflux: 66%, microwave: 98%) and 3b (reflux: 72%, microwave: 97%).The compounds
were analyzed by using Spectrometer FT-Infrared (FT-IR), Spectrometer Nuclear
Magnetic Resonance (NMR) and Mass Spectrometry. Antimicrobial study was also
conducted using Staphylococcus aureus and Bacillus subtillus as
Gram positive, Escherichia coli and Salmonella typhimurium as
Gram negative and fungi Aspergillus niger is used. The positive control used was streptomycin and
kanamycin, while DMSO was used as the negative. However, this study shows no
inhibition of bacteria and fungi (6-7mm of inhibition area) using these
thiourea compounds.
Keywords:
thiourea compound, microvawe, antimicrob activity
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