Malaysian Journal of Analytical Sciences Vol 20 No 2 (2016): 329 - 334

 

 

 

FRACTIONATION OF HEMICELLULOSE FROM RICE STRAW BY ALKALINE EXTRACTION AND ETHANOL PRECIPITATION

 

(Pemisahan Hemiselulosa daripada Hampas Batang Padi melalui Pengekstrakan Beralkali dan Pemendakan Etanol)

 

Nur Hazlilla Zulyadi, Sabiha Hanim Saleh*, Siti Halimah Sarijo

 

School of Chemistry and Environment, Faculty of Applied Sciences,

Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia

 

*Corresponding author: sabihahanim@salam.uitm.edu.my

 

 

Received: 24 February 2015; Accepted: 27 October 2015

 

 

Abstract

Hemicelluloses were extracted from rice straw using alkaline extraction and ethanol precipitation. The effects of different extraction conditions; temperature (45 – 65 °C), pH (4.5 – 6.5), and concentrations of NaOH (0.25 – 1 M) on the hemicellulose yield were investigated. Two hemicelllulosic fractions namely Ha₁ and Ha₂ were obtained by precipitation of alkaline soluble hemicellulose in 0.2 volumes and 4 volumes ethanol, respectively. The Ha₁ and Ha₂ were further characterized to obtain total sugar, monosaccharides and Klason Lignin content. The highest hemicellulose yield for Ha₁ was 4.00 % obtained at 55 °C and pH 5.5 with 0.25 M of NaOH, whereas the highest hemicellulose yield for Ha₂ was 19.88 % obtained at 55 °C and pH 5.5 with 0.5 M of NaOH. This study revealed that hemicellulose yield is dependent on the pH, temperature and concentration of NaOH in the alkaline extraction.

 

Keywords: hemicelluloses, rice straw, alkaline extraction, ethanol precipitation

 

Abstrak

Hemiselulosa daripada hampas batang padi telah diekstrak melalui pengekstrakan beralkali dan pemendakan etanol. Kesan perbezaan kondisi pengekstrakan; suhu (45 – 65 °C), pH (4.5 – 6.5), dan kepekatan NaOH (0.25 – 1 M) ke atas hasil hemiselulosa dikaji. Dua pecahan hemiselulosa,  Ha₁ and Ha₂ telah diperolehi melalui pemendakan hemisellulosa larut alkali dalam isipadu etanol 0.2 dan 4 masing-masing. Ha₁ and Ha₂ dicirikan oleh jumlah gula, kandungan monosakarida dan Klason Lignin. Hasil hemiselulosa tertinggi untuk Ha₁ adalah 4.00 % yang diperolehi pada suhu 55 °C, pH 5.5 and 0.25 M NaOH, manakala untuk Ha₂ adalah 19.88 % diperolehi pada suhu 55 °C, pH 5.5 and kepekatan 0.5 M NaOH. Kajian ini membuktikan hasil hemisellulosa bergantung kepada pH, suhu dan kepekatan NaOH dalam pengekstrakan beralkali.

 

Kata kunci: hemiselulosa, hampas batang padi, pengekstrakan beralkali, pemendakan etanol

 

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