Malaysian
Journal of Analytical Sciences Vol 19 No 5 (2015): 1131 - 1136
GLYCEROL AS A CHEAPER CARBON SOURCE IN BACTERIAL
CELLULOSE (BC) PRODUCTION BY GLUCONACETOBACTER
XYLINUS DSM46604 IN BATCH FERMENTATION SYSTEM
(Gliserol Sebagai Sumber Karbon Kos Rendah Dalam Penghasilan
Bakteria Selulosa (BC) Oleh Gluconacetobacter
xylinus DSM46604 Dalam Sistem Penapaian Berkelompok)
Azila Adnan1*, Giridhar R. Nair2,
Mark C. Lay3, Janis E. Swan3, Roslan Umar4,5
1School of Fundamental Sciences,
Universiti Malaysia
Terengganu, 21030, Kuala Terengganu, Terengganu, Malaysia
2Department of Biotechnology
and Biochemical Engineering,
Sree Buddha College of Engineering, Pattoor P.O., Alappuzha – 690529, India
3School of Engineering, Faculty of Science &
Engineering,
University of
Waikato, Private Bag 3105, Hamilton, New Zealand
4East Coast Environmental Research Institute (ESERI)
5Faculty of Contemporary Islamic Studies
Universiti
Sultan Zainal Abidin, Gong Badak Campus, 21300 Kuala Terengganu, Terengganu,
Malaysia
*Corresponding author: azila.adnan@umt.edu.my
Received:
14 April 2015; Accepted: 9 July 2015
Abstract
Bacterial
cellulose (BC) is a polymer of glucose monomers, which has unique properties
including high crystallinity and high strength. It has potential to be used in
biomedical applications such as making artificial blood vessel, wound
dressings, and in the paper making industry. Extensive study on BC aimed to
improve BC production such as by using glycerol as a cheaper carbon source. BC
was produced in shake flask culture using five different concentrations of
glycerol (10, 20, 30, 40 and 50 g/L). Using concentration of glycerol above 20
g/L inhibited culture growth and BC production. Further experiments were
performed in batch culture (3-L bioreactor) using 20 g/L glycerol. It produced
yield and productivity of 0.15 g/g and 0.29 g/L/day BC, respectively. This is
compared with the control medium, 50 g/L glucose, which only gave yield and
productivity of 0.05 g/g and 0.23 g/L/day, respectively. Twenty g/L of glycerol
enhanced BC production by Gluconacetobacter
xylinus DSM46604 in batch fermentation system.
Keywords: bacterial
cellulose, glycerol, fermentation, carbon source
Abstrak
Bakteria selulosa (BC) adalah polimer daripada monomer glukosa, yang mempunyai ciri-ciri unik termasuk penghabluran yang tinggi dan kekuatan yang tinggi. Ia mempunyai potensi untuk digunakan dalam aplikasi bioperubatan seperti membuat saluran darah tiruan, pembalut luka, dan dalam industri pembuatan kertas itu. Kajian yang menyeluruh pada BC bertujuan untuk meningkatkan pengeluaran BC seperti dengan menggunakan gliserol sebagai sumber karbon kos rendah. Bakteria selulosa telah dihasilkan dalam kelalang penggoncang menggunakan lima kepekatan gliserol yang berbeza (10, 20, 30 , 40 dan 50 g/L). Penggunaan kepekatan gliserol melebihi 20 g /L telah merencat pertumbuhan kultur dan penghasilan BC. Ujikaji lanjutan telah dilakukan di dalam sistem penapaian berkelompok (3-L bioreaktor) menggunakan 20 g/L gliserol. Ia masing-masing telah menghasilkan hasil dan produktiviti sebanyak 0.15 g/g dan 0.29 g/L/hari BC. Ini dibandingkan dengan medium kawalan, 50 g/L glukosa, masing-masing hanya memberikan hasil dan produktiviti sebanyak 0.05 g/g dan 0.23 g/ L/hari BC. Dua puluh g/L gliserol telah mempertingkatkan penghasilan BC oleh Gluconacetobacter xylinus DSM46604 dalam sistem penapaian berkelompok.
Kata kunci: bakteria
selulosa, gliserol, penapaian, sumber karbon
References
1.
Keshk,
S. M. (2014). Vitamin C enhances bacterial cellulose production in Gluconacetobacter xylinus. Carbohydrate
Polymer 99: 98-100.
2.
Mohite,
B. V. and Patil, S. V. (2014). A novel biomaterial: bacterial cellulose and its
new era applications. Biotechnology and Applied Biochemistry 61(2):101 – 110.
3.
El-Saied,
H., Basta, A. H. and Gobran, R. H. (2004). Research Progress in Friendly
Environmental Technology for the Production of Cellulose Products (Bacterial
Cellulose and Its Application). Polymer-Plastics Technology and Engineering 43(3): 797-820.
4.
Mohammadkazemi,
F., Azin, M. and Ashori, A. (2015). Production of bacterial cellulose using
different carbon sources and culture media. Carbohydrate Polymers 117: 518-523.
5.
Koutinas, A. A.,
Sypsas, V., Kandylis, P., Michelis, A., Bekatorou, A., Kourkoutas, Y. and
Yianoulis, P. (2012). Nano-Tubular Cellulose for Bioprocess Technology
Development. PLoS ONE 7(4): 1-9.
6.
Donini, Í. A., De Salvi, D. T., Fukumoto, F.
K., Lustri, W. R., Barud, H. S., Marchetto, R. and Ribeiro, S. J. (2010).
Biosynthesis and recent advances in production of bacterial cellulose. Eclética Química 35(4): 165-178.
7. Johnson, D. T. and Taconi, K. A. (2007). The glycerin
glut: Options for the value-added conversion of crude glycerol resulting from
biodiesel production. Environmental Progress 26(4): 338-348.
8.
Ayoub, M. and
Abdullah, A. Z. (2012). Critical review on the current scenario and
significance of crude glycerol resulting from biodiesel industry towards more
sustainable renewable energy industry. Renewable and Sustainable Energy
Reviews 16(5), 2671-2686.
9.
Miller, G. L. (1959). Use
of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical
Chemistry 31(3): 426–428.
10.
Stasiak-Rozanska,
L., Blazejak, S. and Miklaszewska, A. (2011). Application of immobilized cell
preparation obtained from biomass of Gluconacetobacter
xylinus bacteria in biotransformation of glycerol to dihydroxyacetone. Acta Scientiarum Polonorum. Technologia Alimentaria,
10(1):35-49.
11.
Jung,
H. I., Jeong, J. H., Lee, O. M., Park, G.T., Kim, K. K., Park, H. C., and Son,
H. J. (2010). Influence of glycerol on production and structural–physical
properties of cellulose from Acetobacter
sp. V6 cultured in shake flasks. Bioresource Technology 101(10): 3602-3608.
12.
Kim,
S. Y., Kim, J. N., Wee, Y. J., Park, D. H., and Ryu, H. W. (2006). Production
of bacterial cellulose by Gluconacetobacter
sp. RKY5 isolated from persimmon vinegar. Applied Biochemistry and
Biotechnology 131(1-3),
705-715.
13.
Hungund,
B. S. and Gupta, S. G. (2010). Improved production of bacterial cellulose from Gluconacetobacter persimmonis GH-2. Journal of Microbial and
Biochemical Technology 2(5):
127-133.
14.
Keshk,
S. and Sameshima., K. (2005). Evaluation of different carbon sources for
bacterial cellulose production. African Journal of Biotechnology 4(6): 478-482.
15.
Ross, P., Mayer, P. and Benziman, M. (1991). Cellulose
biosynthesis and function bacteria. Microbiology Review 55: 35-58.
16. Schramm, M.,
Gromet, Z. and Hestrin, S. (1957). Synthesis of cellulose by Acetobacter xylinum. 3. Substrates and
inhibitors. Journal of Biochemistry 67(4): 669-679.