The Malaysian Journal of Analytical Sciences Vol 16 No 2 (2012): 180 – 186

 

 

 

SPECTROPHOTOMETRIC DETERMINATION OF ORGANOPHOSPHATE INSECTICIDE (CHLORPYRIFOS) BASED ON DIAZOTISATION WITH ANTHRANILIC ACID

 

(Penentuan Spektrofotometri Racun Serangga Organofosfat (Chlorpyrifos) Berdasarkan Pendiazoan dengan Asid Antranilik)

 

N.V.S.Venugopal*, B.Sumalatha, Srinivasa Rao Bonthula and G.Veeribabu

 

Department of Chemistry,

G.I.T ,GITAM UNIVERSITY,Visakhapatnam-530045, A.P,India

 

*Corresponding author: venu7000@gmail.com

 

 

Abstract

New reaction system was found for spectrophotometric determination of chlorpyrifos pesticide. This is based on reaction of chlorpyrifos with diazotized anthranilic acid in alkaline medium to form an orange-red color. The solution containing 200ppm chlorpyrifos pesticide and 2 ml of decinormal sodium hydroxide is colorless and with the addition of diazotized anthranilic acid, an immediate orange-red dye is formed. The method is rapid, simple and easy to carry out. The absorbance maximum was observed at 450nm.The Beers law is obeyed up to 8.18 ppm for chlorpyrifos standard solution.  Water and vegetable samples were collected in different areas of Visakhapatnam district, Andhra Pradesh, India to determine the chlorpyrifos and found low levels in the range up to 0.04ppm. Interference study was carried for other pesticides and ions.

 

Keywords: chlorpyrifos, anthranilic acid, diazotization, water and vegetables

 

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